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Title: UVA/UVB-induced genotoxicity and lesion repair in Colossoma macropomum and Arapaima gigas Amazonian fish
Authors: Groff, Aline Aparecida
Silva, J. da
Nunes, Emilene Arusievicz
Ianistcki, Martus
Guecheva, Temenouga Nikolova
Oliveira, Alzira Miranda de
Oliveira, Christiane Patricia Feitosa de
Val, Adalberto Luis
Henriques, João Antonio Pêgas
Keywords: Animals Cell
Animals Experiment
Arapaima Gigas
Colossoma Macropomum
Comet Assay
Concentration Response
Controlled Study
Dna Damage
Dna Repair
Micronucleus Test
Priority Journal
Radiation Exposure
Radiation Injury Repair
Radiation Response
Ultraviolet A Radiation
Ultraviolet B Radiation
Comet Assay
Dna Damage
Dna Repair
Mutagenicity Tests
Ultraviolet Rays
Arapaima Gigas
Colossoma Macropomum
Colossoma Marcopomum
Issue Date: 2010
metadata.dc.publisher.journal: Journal of Photochemistry and Photobiology B: Biology
metadata.dc.relation.ispartof: Volume 99, Número 2, Pags. 93-99
Abstract: Ultraviolet radiation is known to cause adverse effects to aquatic species and aquatic environments. The fish Colossoma macropomum (tambaqui) and Arapaima gigas (pirarucu) live in the Amazon basin, near the Equator, and thus receive high intensity of ultraviolet radiation. Deforestation further aggravates the situation by reducing shade at ground level. The aim of this study was to evaluate the genotoxic effects of UVA and UVB radiation on erythrocytes of tambaqui and pirarucu fish using Micronuclei test and Comet assay. Our study showed that UV radiation caused DNA damage in both species as detected by Comet assay. In addition, there were differences in response to genotoxicity between both species, which are possibly related to their evolutionary history. Tambaqui fish exposed to ultraviolet radiation for different periods presented clear dose-response in DNA damage profile. Significant damage repair was observed 24. h after cessation of ultraviolet radiation exposure. At the test conditions used, no significant increase in micronucleated cells was observed in tambaqui and pirarucu fish. Tambaqui proved to be more sensitive to ultraviolet radiation than Pirarucu, as detected by Comet assay, showing statistically higher baseline DNA damage. The present results demonstrated that alkaline Comet assay was very sensitive for detecting the UV-induced genotoxicity during the short exposure period in our study. In addition, the present study also suggests that tambaqui and pirarucu fish are useful sentinel organisms, as their UV sensitivity allows them to be effective monitors of biological hazards in the Amazon region. © 2010 Elsevier B.V.
metadata.dc.identifier.doi: 10.1016/j.jphotobiol.2010.02.011
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