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Campo DC | Valor | Idioma |
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dc.contributor.author | Moura, Tales R. | - |
dc.contributor.author | Bezerra, Gustavo Arruda | - |
dc.contributor.author | Bezerra, Maria Marques | - |
dc.contributor.author | Teixera, Cícero Silvano | - |
dc.contributor.author | Bezerra, Eduardo Henrique Salviano | - |
dc.contributor.author | Benevides, R. G. | - |
dc.contributor.author | Rocha, Bruno Anderson Matias da | - |
dc.contributor.author | Souza, Luis Augusto Gomes de | - |
dc.contributor.author | Delatorre, Plínio | - |
dc.contributor.author | Nagano, C. S. | - |
dc.contributor.author | Cavada, B. S. | - |
dc.date.accessioned | 2020-06-15T21:55:14Z | - |
dc.date.available | 2020-06-15T21:55:14Z | - |
dc.date.issued | 2009 | - |
dc.identifier.uri | https://repositorio.inpa.gov.br/handle/1/18454 | - |
dc.description.abstract | Plant lectins are the most studied group of carbohydrate-binding proteins. Despite the high similarity between the members of the Diocleinae subtribe (Leguminosae) group, they present differing biological activities. Canavalia boliviana lectin (Cbol) was purified using a Sephadex G-50 column and crystallized in the presence of X-Man by hanging-drop vapour diffusion at 293 K. After optimization, crystals suitable for diffraction were obtained under the condition 0.1 M HEPES pH 7.5 and 3.0 M sodium formate. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 126.70, b = 66.64, c = 64.99 Å, α = 90.0, β = 120.8, γ = 90.0°. Assuming the presence of a dimer in the asymmetric unit, the solvent content was estimated to be about 46%. A complete data set was collected at 1.5 Å resolution. © 2009 International Union of Crystallography All rights reserved. | en |
dc.language.iso | en | pt_BR |
dc.relation.ispartof | Volume 65, Número 3, Pags. 213-215 | pt_BR |
dc.rights | Restrito | * |
dc.subject | Lectin, Canavalia | en |
dc.subject | Plant Lectin | en |
dc.subject | Chromatography, Affinity | en |
dc.subject | Canavalia | en |
dc.subject | Chemistry | en |
dc.subject | Crystallization | en |
dc.subject | Seed Plant | en |
dc.subject | Electrophoresis, Polyacrylamide Gel | en |
dc.subject | X Ray Crystallography | en |
dc.subject | Canavalia | en |
dc.subject | Chromatography, Affinity | en |
dc.subject | Crystallization | en |
dc.subject | Crystallography, X-ray | en |
dc.subject | Electrophoresis, Polyacrylamide Gel | en |
dc.subject | Plant Lectins | en |
dc.subject | Seeds | en |
dc.subject | Canavalia | en |
dc.subject | Canavalia Boliviana | en |
dc.subject | Fabaceae | en |
dc.subject | Piperaceae | en |
dc.title | Crystallization and preliminary X-ray diffraction analysis of the lectin from Canavalia boliviana Piper seeds | en |
dc.type | Artigo | pt_BR |
dc.identifier.doi | 10.1107/S1744309109000797 | - |
dc.publisher.journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications | pt_BR |
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