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Title: Cross-amplification and characterization of microsatellite loci for three species of Theobroma (Sterculiaceae) from the Brazilian Amazon
Authors: Lemes, Maristerra R.
Martiniano, Thieme M.
Reis, Vanessa M.
Faria, Camila P.
Gribel, Rogério
Keywords: Allele
Genetic Differentiation
Molecular Analysis
Genetics, Population
Reproductive Strategy
Theobroma Cacao
Theobroma Grandiflorum
Theobroma Subincanum
Theobroma Sylvestre
Issue Date: 2007
metadata.dc.publisher.journal: Genetic Resources and Crop Evolution
metadata.dc.relation.ispartof: Volume 54, Número 8, Pags. 1653-1657
Abstract: This study reports on the cross-species amplification of 23 microsatellite markers previously developed for Theobroma cacao L. (Sterculiaceae), source of chocolate in three economically important Amazonian species of Theobroma (T. grandiflorum, T. subincanum, T. sylvestre). Thirteen of the 23 microsatellite loci tested were polymorphic across the three species at 2-13 alleles per locus. The observed heterozygosity per locus varied from 0.18 to 0.84 and expected heterozygosity ranged from 0.28 to 0.87. The high level of transferability and genetic information content of these microsatellite loci indicate their usefulness for population genetic, mating system and breeding studies of these economically important Amazonian fruit trees. © 2007 Springer Science+Business Media B.V.
metadata.dc.identifier.doi: 10.1007/s10722-007-9277-7
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