Use este identificador para citar ou linkar para este item:
https://repositorio.inpa.gov.br/handle/1/18656
Registro completo de metadados
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Pohlit, Adrian Martin | - |
dc.contributor.author | Tigre, Ryuga Frota | - |
dc.contributor.author | Coelho Cavalcanti, Bruno | - |
dc.contributor.author | Moraes, Manœl Odorico de | - |
dc.contributor.author | Costa-Lotufo, Leticia Veras | - |
dc.contributor.author | Moraes, Maria Elisabete Amaral de | - |
dc.contributor.author | Santos, Elba Vieira Mustafa dos | - |
dc.contributor.author | Morais, Sabrina Kelly Reis | - |
dc.contributor.author | Nunomura, Sergio Massayoshi | - |
dc.contributor.author | Pessoa, Cláudia Ó. | - |
dc.date.accessioned | 2020-06-15T22:02:27Z | - |
dc.date.available | 2020-06-15T22:02:27Z | - |
dc.date.issued | 2007 | - |
dc.identifier.uri | https://repositorio.inpa.gov.br/handle/1/18656 | - |
dc.description.abstract | This work is part of a larger screening program, which seeks to discover new antitumor plants and compounds from the Brazilian Amazon. In a prescreen of stem and leaf extracts of Tachia grandiflora Maguire & Weaver (Gentianaceae) based on the SRB method, leaf methanol and ethanol extracts showed appreciable cytotoxicity in human breast (MCF-7) and colon (HCT-8) tumor cell lines. Liquid-liquid partitioning of the leaf ethanol extract yielded hexane, chloroform, butanol, and water-methanol fractions. Only the hexane and chloroform fractions were active, inhibiting murine melanoma (B-16) and HCT-8 cells. The chloroform fraction suffered sequential column chromatography on silica gel using different eluent systems and yielded a number of very active subfractions. In all, 25 fractions and subfractions were tested, and 10 exhibited high growth inhibition of HCT-8, and two of these presented strong inhibition of murine melanoma (B-16) cells. The most active subfractions were tested against five tumor cell lines (leukemia CEM and HL-60, as well as the three used previously) using the MTT assay, and four fractions demonstrated significant cytotoxicity based on IC50. Cell lysis was discarded as a possible mechanism for in vitro cytotoxicity given that these fractions did not exhibit hemolytic activity. The greatest antiproliferative potential was found in the second (two samples) and third generation (two samples) chromatographic subfractions of the chloroform fraction (obtained from partitioning of the ethanol extract). These subfractions proved to be complex mixtures from which no pure substance could be isolated after further chromatographic separations. © 2007 Informa Healthcare. | en |
dc.language.iso | en | pt_BR |
dc.relation.ispartof | Volume 45, Número 5, Pags. 429-433 | pt_BR |
dc.rights | Restrito | * |
dc.subject | Alcohol | en |
dc.subject | Butanol | en |
dc.subject | Chloroform | en |
dc.subject | Doxorubicin | en |
dc.subject | Hexane | en |
dc.subject | Methanol | en |
dc.subject | Plant Extract | en |
dc.subject | Silica Gel | en |
dc.subject | Tachia Grandiflora Extract | en |
dc.subject | Unclassified Drug | en |
dc.subject | Water | en |
dc.subject | Animals Cell | en |
dc.subject | Antineoplastic Activity | en |
dc.subject | Breast Tumor | en |
dc.subject | Cancer Inhibition | en |
dc.subject | Cell Strain Hl 60 | en |
dc.subject | Cell Strain Mcf 7 | en |
dc.subject | Colon Tumor | en |
dc.subject | Column Chromatography | en |
dc.subject | Concentration Response | en |
dc.subject | Confidence Interval | en |
dc.subject | Controlled Study | en |
dc.subject | Cytolysis | en |
dc.subject | Drug Activity | en |
dc.subject | Drug Cytotoxicity | en |
dc.subject | Drug Isolation | en |
dc.subject | Drug Research | en |
dc.subject | Drug Screening | en |
dc.subject | Drug Synthesis | en |
dc.subject | Hemolysis | en |
dc.subject | Human | en |
dc.subject | Human Cell | en |
dc.subject | Ic 50 | en |
dc.subject | In Vitro Study | en |
dc.subject | Leukemia | en |
dc.subject | Medicinal Plant | en |
dc.subject | Melanoma B16 | en |
dc.subject | Mouse | en |
dc.subject | Nonhuman | en |
dc.subject | Plant Leaf | en |
dc.subject | Plant Stem | en |
dc.subject | Tachia Grandiflora | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | Gentianaceae | en |
dc.subject | Murinae | en |
dc.subject | Tachia Grandiflora | en |
dc.title | Cytotoxic fractions from the leaves of Tachia grandiflora | en |
dc.type | Artigo | pt_BR |
dc.identifier.doi | 10.1080/13880200701215422 | - |
dc.publisher.journal | Pharmaceutical Biology | pt_BR |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Não existem arquivos associados a este item.
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.