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Título: | Chromosomal differentiation of populations of Lysapsus limellus limellus, L. l. bolivianus, and of Lysapsus caraya (Hylinae, Hylidae) |
Autor: | Busin, Carmen Sílvia Lima, Albertina Pimental Prado, Cynthia P. A. Strüssmann, Christine Júnior, Sérgio Siqueira Pimentel, Shirlei Maria Recco |
Palavras-chave: | Chromosomes Genetic Engineering Morphology Silver Heterochromatin Karyotype Lysapsus Nor Microorganisms Silver Animals Chromosome Chromosome Nor Classification Comparative Study Cytogenetics Diploidy Female In Situ Hybridization, Fluorescence Frogs And Toads Genetics Heterochromatin Karyotyping Male Species Difference Staining Ultrastructure Animal Anura Chromosomes Cytogenetics Diploidy Female Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Male Nucleolus Organizer Region Silver Species Specificity Staining And Labeling Animalsia Hylidae Hylinae Lysapsus Lysapsus Limellus |
Data do documento: | 2006 |
Revista: | Micron |
É parte de: | Volume 37, Número 4, Pags. 355-362 |
Abstract: | Cytogenetic analysis were done on specimens from two populations of Lysapsus limellus limellus, three of L. l. bolivianus and of one of Lysapsus caraya. All animals showed a diploid chromosomal number of 2n=24. The karyotypes of the two L. limellus subspecies were very similar, differing only by the larger amount of telomeric heterochromatin and a small pericentromeric C-band on the short arms of pair 2 in L. l. limellus specimens. The karyotype of L. caraya differed from those of the two L. limellus subspecies in terms of chromosomal morphology, C-banding pattern and location of the main NOR on chromosomes 7 and 6, respectively. The karyotype of the L. l. bolivianus population from Guajará-Mirim/RO differed from those of the other populations of the same subspecies in morphology and heterochromatin pattern of chromosomes 7 and 8. Additional NORs were detected by silver staining and confirmed by FISH in one of the homologues of pairs 1 and 8 in L. l. bolivianus and in pair 7 in L. caraya. These results suggest that a reassessment of the taxonomic status of L. limellus subspecies, especially of the L. l. bolivianus populations, may be necessary. © 2005 Elsevier Ltd. All rights reserved. |
DOI: | 10.1016/j.micron.2005.11.009 |
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