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dc.contributor.authorAdams, Rachel I.-
dc.contributor.authorBrown, Kyle M.-
dc.contributor.authorHamilton, Matthew B.-
dc.date.accessioned2020-06-15T22:03:45Z-
dc.date.available2020-06-15T22:03:45Z-
dc.date.issued2004-
dc.identifier.urihttps://repositorio.inpa.gov.br/handle/1/18894-
dc.description.abstractMicrosatellite allelic states are determined by electrophoretic sizing of polymerase chain reaction fragments to define electromorphs. Numerous studies have documented that identical microsatellite electromorphs are potentially heterogeneous at the DNA sequence level, a phenomenon called electromorph size homoplasy. Few studies have examined the impact of electromorph size homoplasy on estimates of population genetic parameters. We investigated the frequency of microsatellite electromorph size homoplasy for 12 loci in the tropical tree Corythophora alta and 11 loci in the anadromous fish Morone saxatilis by sequencing 14-23 homozygotes per locus sampled from multiple populations for a total of 453 sequences. Sequencing revealed no homoplasy for M. saxatilis loci. Seven C. alta loci exhibited homoplasy, including single and compound repeat motifs both with and without interruptions. Between 12.5 and 42.9% of electromorphs sampled per locus showed size homoplasy. Two methods of correction for homoplasy in C. alta generally produced little or no change in single-locus estimates of RST, except for two loci in which some additional differentiation among populations was revealed. Twelve-locus estimates of R ST (including the seven loci corrected for homoplasy) were slightly greater than estimates from unconnected data, although the 95% confidence intervals overlapped. The frequency of methodological errors such as clerical mistakes or sample mislabelling per genotype scored was estimated at 5.4 and 7.3% for C. alta and M. saxatilis, respectively. Simulations showed that the increase in RST produced by homoplasy correction was only slightly larger than variation in RST estimates expected to be caused by methodological errors.en
dc.language.isoenpt_BR
dc.relation.ispartofVolume 13, Número 9, Pags. 2579-2588pt_BR
dc.rightsRestrito*
dc.subjectMicrosatellite Dnaen
dc.subjectAlleleen
dc.subjectAnimalsen
dc.subjectBassen
dc.subjectComparative Studyen
dc.subjectComputer Simulationen
dc.subjectDna Sequenceen
dc.subjectGenetic Variabilityen
dc.subjectGeneticsen
dc.subjectLecythidaceaeen
dc.subjectMethodologyen
dc.subjectMolecular Geneticsen
dc.subjectNucleotide Sequenceen
dc.subjectPopulation Dynamicsen
dc.subjectGenetics, Populationen
dc.subjectAllelesen
dc.subjectAnimalen
dc.subjectBase Sequenceen
dc.subjectBassen
dc.subjectComputer Simulationen
dc.subjectGenetics, Populationen
dc.subjectLecythidaceaeen
dc.subjectMicrosatellite Repeatsen
dc.subjectMolecular Sequence Dataen
dc.subjectPopulation Dynamicsen
dc.subjectResearch Designen
dc.subjectSequence Analysis, Dnaen
dc.subjectVariation (genetics)en
dc.subjectAnimalsiaen
dc.subjectCorythophoraen
dc.subjectCorythophora Altaen
dc.subjectLecythidaceaeen
dc.subjectMoroneen
dc.subjectMorone Saxatilisen
dc.titleThe impact of microsatellite electromorph size homoplasy on multilocus population structure estimates in a tropical tree (Corythophora alta) and an anadromous fish (Morone saxatilis)en
dc.typeArtigopt_BR
dc.identifier.doi10.1111/j.1365-294X.2004.02256.x-
dc.publisher.journalMolecular Ecologypt_BR
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