Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/36978
Title: Culturable bacteria associated with Anopheles darlingi and their paratransgenesis potential
Authors: Rocha, Elerson Matos
Marinotti, Osvaldo
Serrão, Deidre Machado
Correa, Laura Viana
Katak, Ricardo De Melo
Juan, Campos De Oliveir
Muniz, Veranilce Alves
de Oliveira, Marta Rodrigues
Ferreira, Joaquim
Pessoa, Marcos Cézar Fernandes
Roque, Rosemary Aparecida
da Mota, Adolfo José
Onorati, Piero
Souza-Neto, Jayme A.
Terenius, Olle
Tadei, Wanderli Pedro
Keywords: Amazon forest
Malaria
Microbiota
Mosquito
Vector-borne disease
Issue Date: 2021
metadata.dc.publisher.journal: Malaria Journal
metadata.dc.relation.ispartof: Volume 20, Número 1
Abstract: Background: Malaria remains a major public health problem in South America, mostly in the Amazon region. Among newly proposed ways of controlling malaria transmission to humans, paratransgenesis is a promising alternative. Paratransgenesis aims to inhibit the development of parasites within the vector through the action of genetically modified bacteria. The first step towards successful paratransgenesis in the Amazon is the identification of Anopheles darlingi symbiotic bacteria, which are transmitted vertically among mosquitoes, and are not pathogenic to humans. Methods: Culturable bacteria associated with An. darlingi and their breeding sites were isolated by conventional microbiological techniques. Isolated strains were transformed with a GFP expressing plasmid, pSPT-1-GFP, and reintroduced in mosquitoes by feeding. Their survival and persistence in the next generation was assessed by the isolation of fluorescent bacteria from eggs, larvae, pupae and adult homogenates. Results: A total of 179 bacterial strains were isolated from samples from two locations, Coari and Manaus. The predominant genera identified in this study were Acinetobacter, Enterobacter, Klebsiella, Serratia, Bacillus, Elizabethkingia, Stenotrophomonas and Pantoea. Two isolated strains, Serratia-Adu40 and Pantoea-Ovo3, were successfully transformed with the pSPT-1-GFP plasmid and expressed GFP. The fluorescent bacteria fed to adult females were transferred to their eggs, which persisted in larvae and throughout metamorphosis, and were detected in adult mosquitoes of the next generation. Conclusion: Serratia-Adu40 and Pantoea-Ovo3 are promising candidates for paratransgenesis in An. darlingi. Further research is needed to determine if these bacteria are vertically transferred in nature. © 2021, The Author(s).
metadata.dc.identifier.doi: 10.1186/s12936-020-03574-1
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