Please use this identifier to cite or link to this item:
Title: Pathological findings and morphologic correlation of the lungs of autopsied patients with sars-cov-2 infection in the Brazilian Amazon using transmission electron microscopy
Authors: Santana, Monique Freire
Pinto, Rebecca Augusta De Araújo
Marcon, Bruna Hilzendeger
Medeiros, Lia Carolina Almeida Soares de
Morais, Thiago Barros Do Nascimento de
Dias, Lucas Castanhola
Souza, Lorenna Pereira de
Melo, Gisely Cardoso de
Monteiro, Wuelton Marcelo
Lacerda, Marcus V. G.
Val, Fernando Almeida
Lalwani, Pritesh
Ferreira, Luís Carlos De Lima
Keywords: COVID-19
Diffuse alveolar damage
Electron microscopy
Lung pathology
Postmortem evidence
Severe acute respiratory syndrome 2
Issue Date: 2021
metadata.dc.publisher.journal: Revista da Sociedade Brasileira de Medicina Tropical
metadata.dc.relation.ispartof: Volume 54; Number e0850-2020
Abstract: Introduction: Electron microscopy (EM) is a rapid and effective tool that can be used to create images of a whole spectrum of virus-host interactions and, as such, has long been used in the discovery and description of viral mechanisms. Methods: Electron microscopy was used to evaluate the pulmonary pathologies of postmortem lung sections from three patients who died from infection with SARS-associated coronavirus 2 (SARS-CoV-2), a new member of the Coronaviridae family. Results: Diffuse alveolar damage (DAD) was predominant in all three patients. The early exudative stage was characterized principally by edema and extravasation of red blood cells into the alveolar space with injury to the alveolar epithelial cells; this was followed by detachment, apoptosis, and necrosis of type I and II pneumocytes. The capillaries exhibited congestion, exposure of the basement membrane from denuded endothelial cells, platelet adhesion, fibrin thrombi, and rupture of the capillary walls. The proliferative stage was characterized by pronounced proliferation of type II alveolar pneumocytes and multinucleated giant cells. The cytopathic effect of SARS-CoV-2 was observed both in degenerated type II pneumocytes and freely circulating in the alveoli, with components from virions, macrophages, lymphocytes, and cellular debris. Conclusions: Viral particles consistent with the characteristics of SARS-CoV-2 were observed mainly in degenerated pneumocytes, in the endothelium, or freely circulating in the alveoli. In the final stage of illness, the alveolar spaces were replaced by fibrosis. © 2021, Sociedade Brasileira de Medicina Tropical. All rights reserved.
metadata.dc.identifier.doi: 10.1590/0037-8682-0850-2020
Appears in Collections:Artigos

Files in This Item:
File Description SizeFormat 
Pathological finding.pdf60,92 kBAdobe PDFView/Open

This item is licensed under a Creative Commons License Creative Commons