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Campo DC | Valor | Idioma |
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dc.contributor.author | Morais, Francimauro Sousa | - |
dc.contributor.author | Canuto, Kirley Marques | - |
dc.contributor.author | Ribeiro, Paulo Riceli Vasconcelos | - |
dc.contributor.author | Silva, Alison Batista da | - |
dc.contributor.author | Pessoa, Otília Deusdênia Loiola | - |
dc.contributor.author | Freitas, Cleverson Diniz Teixeira de | - |
dc.contributor.author | Bezerra, Emanuel Alves | - |
dc.contributor.author | Gonçalves, José F. C. | - |
dc.contributor.author | Souza, Diego Pereira De | - |
dc.contributor.author | Sousa, Brandon Ferraz E. | - |
dc.contributor.author | Silva, Ayrles Fernanda Brandão | - |
dc.contributor.author | Ramos, Márcio Viana | - |
dc.date.accessioned | 2021-06-21T19:42:51Z | - |
dc.date.available | 2021-06-21T19:42:51Z | - |
dc.date.issued | 2021 | - |
dc.identifier.uri | https://repositorio.inpa.gov.br/handle/1/37772 | - |
dc.description.abstract | Vigna unguiculata is an important source of proteins and energy for humans and animals. However, postharvest losses caused by Callosobruchus maculatus can reach from 20 to 100% of stored seeds. In this study, the insecticide potential of compounds extracted from Himatanthus drasticus latex was assessed. The latex was extracted with ethanol (70%) and then partitioned through sequential use of hexane and chloroform. These fractions were investigated by chromatography to determine their chemical composition. Plumieride, identified in a hydroalcoholic subfraction, was tested for insecticidal activity against C. maculatus. The ethanolic fraction (LC50 = 0.109; LC90 = 0.106%) and the plumieride (LC50 = 0.166; LC90 = 0.167%) were lethal to larvae. Plumieride (0.25%) delayed larval development, and mortality reached 100%. Its inhibitory action on intestinal α-Amylase from larvae was higher (89.12%) than that of acarbose (63.82%). Plumieride (0.1%) inhibited the enzyme α-Amylase in vivo in the larval intestine. This result was confirmed by a zymogram test performed by SDS-PAGE when the enzyme electrophoresed on gel copolymerized with starch. When spread on seeds, the hydroalcoholic fraction (1.0%) reduced infestation. The loss of seed mass was 5.26% compared to the control (44.97%). The results confirm the effect of latex compounds in protecting stored seeds against weevil infestation. | pt_BR |
dc.language.iso | en | pt_BR |
dc.relation.ispartof | Volume 69; págs.: 5049-5058 | pt_BR |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Brazil | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/br/ | * |
dc.subject | Antimetabolic plumieride | pt_BR |
dc.subject | Insect control | pt_BR |
dc.subject | Latex | pt_BR |
dc.subject | Plant defense | pt_BR |
dc.subject | Seed storage | pt_BR |
dc.title | Insecticidal Compound from Himatanthus drasticus Latex against Cowpea Infestation by Callosobruchus maculatus (Coleoptera: Chrysomelidae) | pt_BR |
dc.type | Artigo | pt_BR |
dc.identifier.doi | 10.1021/acs.jafc.1c01177 | - |
dc.publisher.journal | Journal of Agricultural and Food Chemistry | pt_BR |
Aparece nas coleções: | Artigos IPUB |
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Arquivo | Descrição | Tamanho | Formato | |
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Insecticidal_Compound.pdf | 10,18 MB | Adobe PDF | Visualizar/Abrir |
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