Use este identificador para citar ou linkar para este item:
https://repositorio.inpa.gov.br/handle/1/37784
Registro completo de metadados
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Marques, Gabriela Fernandes Oliveira | - |
dc.contributor.author | Pires, Alana De Freitas | - |
dc.contributor.author | Osterne, Vinicius J.S. | - |
dc.contributor.author | Pinto-Junior, Vanir Reis | - |
dc.contributor.author | Silva, Ivanice Bezerra | - |
dc.contributor.author | Martins, Maria Gleiciane Queiroz | - |
dc.contributor.author | Oliveira, Messias Vital | - |
dc.contributor.author | Gomes, Angélica Maciel | - |
dc.contributor.author | Souza, Luiz Augusto Gomes de | - |
dc.contributor.author | Pavão, M. S. | - |
dc.contributor.author | Cavada, B. S. | - |
dc.contributor.author | Assreuy, Ana Maria Sampaio | - |
dc.contributor.author | Nascimento, K. S. | - |
dc.date.accessioned | 2021-06-25T19:49:59Z | - |
dc.date.available | 2021-06-25T19:49:59Z | - |
dc.date.issued | 2021 | - |
dc.identifier.uri | https://repositorio.inpa.gov.br/handle/1/37784 | - |
dc.description.abstract | Using a rat model of peritonitis, we herein report the inflammatory effect induced by the lectin isolated from Vatairea guianensis (VGL) seeds in the context of interactions between VGL and both toll-like receptor 4 (TLR4) and tumor necrosis factor receptor 1 (TNFR1). Peritoneal macrophages were stimulated with VGL for dose-dependent gene expression and release of TNF-α. In vivo results showed that VGL (1 mg/kg; intraperitoneal) induced peritonitis in female Wistar rats. Leukocyte migration, macrophage activation, and protein leakage were measured 3 and 6 hours after induction. In vitro, peritoneal macrophages were stimulated with VGL for gene expression and TNF-α dosage (mean ± SEM (n = 6), analysis of variance, and Bonferroni's test (P <.05)). In silico, VGL structure was applied in molecular docking with representative glycans. It was found that (a) VGL increases vascular permeability and stimulates leukocyte migration, both rolling and adhesion; (b) lectin-induced neutrophil migration occurs via macrophage stimulation, both in vitro and in vivo; (c) lectin interacts with TLR4 and TNFR1; and (d) stimulates TNF-α gene expression (RT-PCR) and release from peritoneal macrophages. Thus, upon lectin-glycan binding on the cell surface, our results suggest that VGL induces an acute inflammatory response, in turn activating the release of peritoneal macrophages via TNF-α and TLR and/or TNFR receptor pathways. © 2021 John Wiley & Sons Ltd. | en |
dc.title | Vatairea guianensis lectin stimulates changes in gene expression and release of TNF-α from rat peritoneal macrophages via glycoconjugate binding | pt_BR |
dc.type | Artigo | pt_BR |
dc.identifier.doi | 10.1002/jmr.2922 | - |
dc.publisher.journal | Journal of Molecular Recognition | en |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Não existem arquivos associados a este item.
Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.