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Title: Larvicidal activities against aedes aegypti of supernatant and pellet fractions from cultured bacillus spp. Isolated from amazonian microenvironments
Authors: Katak, Ricardo De Melo
Rocha, Elerson Matos
Oliveira, Juan C.
Muniz, Veranilce Alves
Oliveira, Marta R.
Ferreira, Francisco Augusto Da Silva
Silva, William Ribeiro Da
Roque, Rosemary Aparecida
de Souza, Antônia Queiroz Lima
Souza-Neto, Jayme A.
Terenius, Olle
Marinotti, Osvaldo
Tadei, Wanderli Pedro
Issue Date: 2021
metadata.dc.publisher.journal: Tropical Medicine and Infectious Disease
metadata.dc.relation.ispartof: Volume 6
Abstract: The Aedes aegypti mosquito is the primary vector of Dengue, Chikungunya and Zika causing major problems for public health, which requires new strategies for its control, like the use of entomopathogenic microorganisms. In this study, bacteria from various Amazonian environments were isolated and tested for their pathogenicity to A. aegypti larvae. Following thermal shock to select sporulated Bacillus spp., 77 bacterial strains were isolated. Molecular identification per 16S RNA sequences revealed that the assembled strains contained several species of the genus Bacillus and one species each of Brevibacillus, Klebsiella, Serratia, Achromobacter and Brevundimonas. Among the isolated Bacillus sp. strains, 19 showed larvicidal activity against A. aegypti. Two strains of Brevibacillus halotolerans also displayed larvicidal activity. For the first time, larvicidal activity against A. aegypti was identified for a strain of Brevibacillus halotolerans. Supernatant and pellet fractions of bacterial cultures were tested separately for larvicidal activities. Eight strains contained isolated fractions resulting in at least 50% mortality when tested at a concentration of 5 mg/mL. Further studies are needed to characterize the active larvicidal metabolites produced by these microorganisms and define their mechanisms of action. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
metadata.dc.identifier.doi: 10.3390/TROPICALMED6020104
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