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https://repositorio.inpa.gov.br/handle/1/37977
Título: | Highly sensitive electrochemical immunosensor using a protein-polyvinylidene fluoride nanocomposite for human thyroglobulin |
Autor: | Moraes, Maria Oneide Silva de de Moraes Segundo, João Deus Pereira Paula, Marcos Marques Da Silva Sales, Maria Goreti Ferreira Brito, W. R. |
Palavras-chave: | Electrochemical immunosensor Point-of-care PVDF nanocomposites, gold nanoparticles Thyroglobulin |
Data do documento: | 2021 |
Revista: | Bioelectrochemistry |
É parte de: | Volume 142, Número 142 |
Abstract: | The highly sensitive detection of serum thyroglobulin (Tg) is essential in the post-treatment follow-up of patients with differentiated thyroid cancer undergoing total or partial thyroidectomy and radioactive iodine ablation and requires sensitive, accurate and stable methods. This work proposes an electrochemical immunosensor for the detection of serum Tg antigen, making use of innovative nanocomposites including polyvinylidene fluoride (PVDF) microparticles coated with streptavidin (MP) and gold nanoparticles (AuNPs). The functionalized polymer matrices were characterized by UV–Vis, FTIR, XPS, SEM, dynamic light scattering, and free surface energy. Immobilization of biotin-labeled anti-thyroglobulin monoclonal antibodies was achieved by binding these to the polymer nanocomposite via streptavidin proteins. The analytical response was measured in quintuplicate and had a linear profile from 2.0 to 10.0 ng/mL Tg, with r2 of 0.985. The limits of detection and quantification were excellent, equal to 0.015 and 0.047 ng/mL, respectively. In addition, the recovery factor was equal to 95.4% (1.0 ng/mL Tg). Overall, the innovative polymer-based nanocomposite used herein enabled the production of an electrochemical-based immunosensor with excellent sensitivity, selectivity, and reproducibility. It evidenced the remarkable potential of determining low levels of Tg in in vitro assays, thereby suggesting that it may be considered for the analyzes of serum patients. © 2021 Elsevier B.V. |
DOI: | 10.1016/j.bioelechem.2021.107888 |
Aparece nas coleções: | Artigos |
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