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dc.contributor.authorAntonelli, Alexandre-
dc.contributor.authorBacon, Christine D.-
dc.contributor.authorGroppo, Milton-
dc.contributor.authorAndermann, Tobias-
dc.contributor.authorBatista, Romina-
dc.contributor.authorFerreira, Paola De Lima-
dc.date.accessioned2022-02-25T21:09:56Z-
dc.date.available2022-02-25T21:09:56Z-
dc.date.issued2022-
dc.identifier.urihttps://repositorio.inpa.gov.br/handle/1/38479-
dc.description.abstractTarget sequence capture has emerged as a powerful method to sequence hundreds or thousands of genomic regions in a cost- and time-efficient approach. In most cases, however, targeted regions lack full sequence information for certain samples, due to taxonomic, laboratory, or stochastic factors. Loci lacking molecular data for a large number of samples are commonly excluded from downstream analyses, even though they may still contain valuable information. On the other hand, including data-poor loci may bias phylogenetic analyses. Here we use a target sequence capture dataset of an ecologically and taxonomically diverse group of spiny sunflowers (Asteraceae, or Compositae: Barnadesioideae) to test how the inclusion or exclusion of such data-poor loci affects phylogenetic inference. We investigate the sensitivity of concatenation and coalescent approaches to missing data with matrices of varying taxonomic completeness by filtering loci with different proportions of missing samples prior to data analysis. We find that missing data affect both the topology and branch support of the resulting phylogenies. The matrix containing all loci yielded the overall highest node support values, independently of the amount of missing nucleotides. These results provide empirical support to earlier suggestions based on single genes and data simulations that taxa with high amounts of missing data should not be readily dismissed as they can provide essential information for phylogenomic reconstruction. © 2022 The Authorspt_BR
dc.language.isoenpt_BR
dc.relation.ispartofVolume 169, Número 107432pt_BR
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/br/*
dc.subjectHigh-throughput sequencingpt_BR
dc.subjectMissing datapt_BR
dc.titleTarget sequence capture of Barnadesioideae (Compositae) demonstrates the utility of low coverage loci in phylogenomic analysespt_BR
dc.typeArtigopt_BR
dc.identifier.doi10.1016/j.ympev.2022.107432-
dc.publisher.journalMolecular Phylogenetics and Evolutionpt_BR
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