Enzymatic variability in Aedes aegypti (Diptera: Culicidae) populations from Manaus-AM, Brazil

Abstract

Eighteen enzymatic loci were analysed in Aedes aegypti populations from four neighbourhoods in the city of Manaus. The analyses showed that the Downtown population was the most polymorphic (p = 55.6%) with higher observed and expected mean heterozygosities (Ho = 0.152 ± 0.052; He = 0.174 ± 0.052, respectively. The least variability was detected in the Coroado and Cidade Nova populations, both with polymorphism of 44.4%. The latter population presented the least observed heterozygosity (Ho = 0.109 ± .037). Wright's F statistics showed that the mean value of Fis as higher than that of Fst (Fis = 0. 164 > Fst = 0.048), and from analysis of molecular variance (AMOVA) it was found that 95.12% of the variability is found within populations indicating a certain intra-population differentiation possibly of the microgeographic structure resulting from some barrier in the random coupling. Although the four populations were similar genetically (D = 0.003 to 0.016), the 4.88% differentiation was significant.