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Título: | Isoenzymatic Variability among Five Anopheles Species Belonging to the Nyssorhynchus and Anopheles Subgenera of the Amazon Region, Brazil |
Autor: | Santos, Joselita Maria Mendes dos Maia, Juracy de Freitas Tadei, Wanderli Pedro Rodriguez, Gloria Alicia Diaz |
Palavras-chave: | Isoenzyme Allele Animals Experiment Anopheles Controlled Study Genetic Distance Genetic Variability Heterozygosity Morphology Nonhuman Phylogeny Polymorphic Locus Animals Classification Disease Carrier Enzymology Gene Genetic Variability Genetics Species Difference Albitarsis Animalsia Nyssorhynchus Alleles Animal Anopheles Genes, Insect Insect Vectors Isoenzymes Species Specificity Variation (genetics) |
Data do documento: | 2003 |
Revista: | Memórias do Instituto Oswaldo Cruz |
É parte de: | Volume 98, Número 2, Pags. 247-253 |
Abstract: | An isoenzymatic comparative analysis of the variability and genetics differentiation among Anopheles species was done in populations of An. (Nys.) intermedius and An. (Ano.) mattogrossensis of the Anopheles subgenus, and of An. darlingi, An. albitarsis and An. triannulatus of the Nyssorhynchus subgenus, with the aim of detecting differences between both subgenera and of estimating the degree of genetic interespecific divergence. Samples from Macapá, State of Amapá and Janauari Lake, near Manaus, State of Amazonas, were analyzed for eight isoenzymatic loci. Analysis revealed differences in the average number of alleles per locus (1.6-2.3) and heterozygosity (0.060-0.284). However, the proportion of polymorphic loci was the same for An. (Nys.) darlingi, An. (Nys.) triannulatus and An. (Ano.) mattogrossensis (50%), but differed for An. (Nys.) albitarsis (62.5%) and An. (Ano.) intermedius (25%). Only the IDH1 (P > 0.5) locus in all species studied was in Hardy-Weinberg equilibrium. The fixation index demonstrated elevated genetic structuring among species, based on values of Fst = 0.644 and genetic distance (0.344-0.989). Genetic difference was higher between An. (Nys.) triannulatus and An. (Ano.) intermedius (0.989) and smaller between An. (Nys.) albitarsis sensu lato and An. (Nys.) darlingi (0.344). The data show interspecific genetic divergence which differs from the phylogenetic hypothesis based on morphological characters. |
DOI: | 10.1590/S0074-02762003000200014 |
Aparece nas coleções: | Artigos |
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