Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/14568
Title: Characterization of Endotrypanum Parasites Using Specific Monoclonal Antibodies
Authors: Franco, A. M.
MacHado, Gérzia Maria de Carvalho
Naiff, Roberto Daibes
Moreira, Celia F.S.
McMahon-Pratt, Diane
Grimaldi Júnior, Gabriel F.
Keywords: Edentata
Endotrypanum
Endotrypanum Schaudinni
Kinetoplastida
Leishmania Equatorensis
Leishmania Major
Leishmania Tropica
Mammalia
Rodentia
Trypanosomatidae
Issue Date: 1997
metadata.dc.publisher.journal: Memórias do Instituto Oswaldo Cruz
metadata.dc.relation.ispartof: Volume 92, Número 1, Pags. 63-68
Abstract: A large number of Endotrypanum stocks (representing an heterogeneous population of strains) have been screened against a panel of monoclonal antibodies (MAbs) derived for selected species of Endotrypanum or Leishmania, to see whether this approach could be used to group/differentiate further among these parasites. Using different immunological assay systems, MAbs considered specific for the genus Endotrypanum (E-24, CXXX-3G5-F12) or strain M6159 of E. schaudinni (E-2. CXIV-3C7-F5) reacted variably according to the test used but in the ELISA or immunofluorescence assay both reacted with all the strains tested. Analyses using these MAbs showed antigenic diversity occurring among the Endotrypanum strains, but no qualitative or quantitative reactivity pattern could be consistently related to parasite origin (i.e., host species involved) or geographic area of isolation. Western blot analyses of the parasites showed that these MAbs recognized multiple components. Differences existed either in the epitope density or molecular forms associated with the antigenic determinants and therefore allowed the assignment of the strains to specific antigenic groups. Using immunofluorescence or ELISA assay, clone E-24 produced reaction with L. equatorensis (which is a parasite of sloth and rodent), but not with other trypanosomatids examined. Interestingly, the latter parasite and the Endotrypanum strains cross-reacted with a number of MAbs that were produced against members of the L. major-L. tropica complex.
metadata.dc.identifier.doi: 10.1590/S0074-02761997000100013
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