Please use this identifier to cite or link to this item:
Title: Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
Authors: Val, Vera Maria Fonseca Almeida e
Oliveira, Alice Reis
Silva, Maria de Nazaré Paula da
Ferreira-Nozawa, Mônica Stropa
Araújo, Roziete Mendes
Val, Adalberto Luis
Nozawa, Sérgio Ricardo
Keywords: Lactate Dehydrogenase
Lactate Dehydrogenase Isoenzyme 5
Rna, Messenger
Unclassified Drug
Animals Experiment
Animals Tissue
Astronotus Crassipinis
Controlled Study
Gene Expression
Gene Sequence
Heart Muscle
Ldh A Gene
Cloning, Molecular
Nucleotide Sequence
Muscle, Skeletal
Issue Date: 2011
metadata.dc.publisher.journal: Genetics and Molecular Biology
metadata.dc.relation.ispartof: Volume 34, Número 2, Pags. 315-322
Abstract: Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such aserythropoietin,transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis(the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A expression. In adults, the levels of LDH-A mRNA did not increase in hypoxic or anoxic conditions. Our results demonstrate that, when given time for acclimation, fish at different life-stages are able to respond differently to survive hypoxic episodes. © 2011, Sociedade Brasileira de Genética.
metadata.dc.identifier.doi: 10.1590/S1415-47572011000200025
Appears in Collections:Artigos

Files in This Item:
File Description SizeFormat 
artigo-inpa.pdf1,77 MBAdobe PDFThumbnail

This item is licensed under a Creative Commons License Creative Commons