Use este identificador para citar ou linkar para este item: https://repositorio.inpa.gov.br/handle/1/14955
Título: Anoxia- and hypoxia-induced expression of LDH-A* in the Amazon Oscar, Astronotus crassipinis
Autor: Val, Vera Maria Fonseca Almeida e
Oliveira, Alice Reis
Silva, Maria de Nazaré Paula da
Ferreira-Nozawa, Mônica Stropa
Araújo, Roziete Mendes
Val, Adalberto Luis
Nozawa, Sérgio Ricardo
Palavras-chave: Lactate Dehydrogenase
Lactate Dehydrogenase Isoenzyme 5
Rna, Messenger
Unclassified Drug
Acclimatization
Age
Animals Experiment
Animals Tissue
Anoxia
Astronotus Crassipinis
Controlled Study
Fish
Gene Expression
Gene Sequence
Heart Muscle
Hypoxia
Ldh A Gene
Cloning, Molecular
Nonhuman
Nucleotide Sequence
Muscle, Skeletal
Astronotus
Pisces
Data do documento: 2011
Revista: Genetics and Molecular Biology
Encontra-se em: Volume 34, Número 2, Pags. 315-322
Abstract: Adaptation or acclimation to hypoxia occurs via the modulation of physiologically relevant genes, such aserythropoietin,transferrin, vascular endothelial growth factor, phosphofructokinase and lactate dehydrogenase A. In the present study, we have cloned, sequenced and examined the modulation of the LDH-A gene after an Amazonian fish species, Astronotus crassipinis(the Oscar), was exposed to hypoxia and anoxia. In earlier studies, we have discovered that adults of this species are extremely tolerant to hypoxia and anoxia, while the juveniles are less tolerant. Exposure of juveniles to acute hypoxia and anoxia resulted in increased LDH-A gene expression in skeletal and cardiac muscles. When exposed to graded hypoxia juveniles show decreased LDH-A expression. In adults, the levels of LDH-A mRNA did not increase in hypoxic or anoxic conditions. Our results demonstrate that, when given time for acclimation, fish at different life-stages are able to respond differently to survive hypoxic episodes. © 2011, Sociedade Brasileira de Genética.
DOI: 10.1590/S1415-47572011000200025
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