Use este identificador para citar ou linkar para este item:
https://repositorio.inpa.gov.br/handle/1/15002
Título: | Identification of Colletotrichum isolates from Capsicum chinense in Amazon |
Autor: | Oliveira, C. V.S. de Matos, Kedma Silva Albuquerque, Dulcinéia Martins C. de Hanada, Rogério Eiji Silva, Gilvan Ferreira da |
Palavras-chave: | Actin Glyceraldehyde 3 Phosphate Dehydrogenase Molecular Marker Superoxide Dismutase Amazonas Capsicum Chinense Colletotrichum Colletotrichum Brevisporum Colletotrichum Scovillei Colletotrichum Siamense Controlled Study Crop Production Fungus Identification Fungus Isolation Inter Simple Sequence Repeat Nonhuman Nucleotide Sequence Phylogeny Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Unindexed Sequence |
Data do documento: | 2017 |
Revista: | Genetics and Molecular Research |
É parte de: | Volume 16, Número 2 |
Abstract: | Chili pepper (Capsicum chinense) is a great economic important culture on the State of Amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. It is one of the hosts of Colletotrichum genus in the North region of Brazil. The aim of the study was to differentiate and to identify isolates of Colletotrichum collected from C. chinense in Amazon. Molecular characterization, using RFLP-PCR, ERIC-PCR and ISSR, was carried out initially for screening of morphologically similar isolates. Furthermore, phylogenetic analyses were performed using combined regions: Actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for the three isolates, INPA 2066, INPA 2286 and INPA 1858, plus superoxide dismutase (SOD2) for INPA 2066. We showed that the molecular markers were able to distinguish the isolates of Colletotrichum studied and these results were confirmed with the phylogenetic analyses, three different occurrences of Colletotrichum species (C. siamense, C. scovillei and C. brevisporum) causing anthracnose in C. chinense in the State of Amazonas. This study represents the first report of the species C. siamense and C. scovillei in this host in Brazil. © 2017 The Authors. |
DOI: | 10.4238/gmr16029601 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
artigo-inpa.pdf | 2,24 MB | Adobe PDF | Visualizar/Abrir |
Este item está licenciada sob uma Licença Creative Commons