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Title: Fluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridization
Authors: Silva, H. C.M.
Martins-Júnior, M. M.C.
Braga Ribeiro, Leila
Matoso, D. A.
Keywords: Biotin
Controlled Study
In Situ Hybridization, Fluorescence
Image Analysis
Quantitative Analysis
Evaluation Study
In Situ Hybridization, Fluorescence
Microscopy, Fluorescence
Image Processing
Nucleic Acid Hybridization
Evaluation Studies As Topic
Image Processing, Computer-assisted
In Situ Hybridization, Fluorescence
Microscopy, Fluorescence
Nucleic Acid Hybridization
Issue Date: 2017
metadata.dc.publisher.journal: Genetics and Molecular Research
metadata.dc.relation.ispartof: Volume 16, Número 1
Abstract: The Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLIMA software calculates the amount of pixels on image and recognizes each present color. The coefficient generated by the algorithm shows the percentage of marks (probes) hybridized on the chromosomes. This software can be used for any type of image generated by a fluorescence microscope and is able to quantify digoxigenin probes exhibiting a red color, biotin probes exhibiting a green color, and double-FISH probes (digoxigenin and biotin used together), where the white color is displayed. © 2017 The Authors.
metadata.dc.identifier.doi: 10.4238/gmr16019658
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