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dc.contributor.authorSilva, H. C.M.-
dc.contributor.authorMartins-Júnior, M. M.C.-
dc.contributor.authorBraga Ribeiro, Leila-
dc.contributor.authorMatoso, D. A.-
dc.date.accessioned2020-05-07T14:00:36Z-
dc.date.available2020-05-07T14:00:36Z-
dc.date.issued2017-
dc.identifier.urihttps://repositorio.inpa.gov.br/handle/1/15017-
dc.description.abstractThe Fluorescence Image Analyzer (FLIMA) software was developed for the quantitative analysis of images generated by fluorescence in situ hybridization (FISH). Currently, the images of FISH are examined without a coefficient that enables a comparison between them. Through GD Graphics Library, the FLIMA software calculates the amount of pixels on image and recognizes each present color. The coefficient generated by the algorithm shows the percentage of marks (probes) hybridized on the chromosomes. This software can be used for any type of image generated by a fluorescence microscope and is able to quantify digoxigenin probes exhibiting a red color, biotin probes exhibiting a green color, and double-FISH probes (digoxigenin and biotin used together), where the white color is displayed. © 2017 The Authors.en
dc.language.isoenpt_BR
dc.relation.ispartofVolume 16, Número 1pt_BR
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Brazil*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/br/*
dc.subjectBiotinen
dc.subjectDigoxigeninen
dc.subjectAlgorithmen
dc.subjectChromosomeen
dc.subjectColoren
dc.subjectControlled Studyen
dc.subjectIn Situ Hybridization, Fluorescenceen
dc.subjectImage Analysisen
dc.subjectQuantitative Analysisen
dc.subjectEvaluation Studyen
dc.subjectIn Situ Hybridization, Fluorescenceen
dc.subjectMicroscopy, Fluorescenceen
dc.subjectImage Processingen
dc.subjectNucleic Acid Hybridizationen
dc.subjectProceduresen
dc.subjectSoftwareen
dc.subjectChromosomesen
dc.subjectEvaluation Studies As Topicen
dc.subjectImage Processing, Computer-assisteden
dc.subjectIn Situ Hybridization, Fluorescenceen
dc.subjectMicroscopy, Fluorescenceen
dc.subjectNucleic Acid Hybridizationen
dc.subjectSoftwareen
dc.titleFluorescence image analyzer - FLIMA: Software for quantitative analysis of fluorescence in situ hybridizationen
dc.typeArtigopt_BR
dc.identifier.doi10.4238/gmr16019658-
dc.publisher.journalGenetics and Molecular Researchpt_BR
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