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Título: | Coetzeea brasiliensis gen. Nov., sp. nov. isolated from larvae of Anopheles darlingi |
Autor: | Kämpfer, Peter Glaeser, Stefanie P. Marinotti, Osvaldo Guy, Lionel Håkansson, Sebastian Tadei, Wanderli Pedro Busse, Hans Jürgen Terenius, Olle |
Palavras-chave: | Dna, Bacterial Cardiolipin Fatty Acid Nucleotide Phosphatidylglycerol Polyamine Putrescine Rna 16s Ubiquinone Dna, Bacterial Fatty Acid Phospholipid Putrescine Rna 16s Ubiquinone Ubiquinone 8 Anopheles Darlingi Bacterium Isolation Chemotaxonomy Coetzeea Brasiliensis Dna Hybridization Fatty Acid Analysis Gene Sequence Gram Negative Bacterium Larva Multilocus Sequence Typing Nonhuman Phylogenetic Tree Phylogeny Priority Journal Thorsellia Anophelis Thorsellia Kandunguensis Thorsellia Kenyensis Type Strain Animals Anopheles Bacterium Identification Chemistry Classification Dna Base Composition Dna Sequence Enterobacteriaceae Genetics Isolation And Purification Larva Microbiology Nucleic Acid Hybridization Phylogeny Animal Anopheles Bacterial Typing Techniques Base Composition Dna, Bacterial Enterobacteriaceae Fatty Acids Larva Nucleic Acid Hybridization Phospholipids Phylogeny Putrescine Rna, Ribosomal, 16s Sequence Analysis, Dna Ubiquinone |
Data do documento: | 2016 |
Revista: | International Journal of Systematic and Evolutionary Microbiology |
É parte de: | Volume 66, Número 12, Pags. 5211-5217 |
Abstract: | A Gram-stain-negative, rod-shaped strain, Braz8T, isolated from larvae of Anopheles darlingi was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Braz8T was related most closely to species of the genus Thorsellia, with 95.6, 96.5 and 96.6% similarity to the type strains of Thorsellia anophelis, Thorsellia kandunguensis and Thorsellia kenyensis, respectively, and formed a separate branch in the phylogenetic tree next to the monophyletic cluster of the genus Thorsellia. Chemotaxonomic data supported the allocation of the strain to the family Thorselliaceae. The major fatty acids were C18:1ω7c, C16:0 and C14:0. The quinone system was composed of ubiquinones Q-8 and Q-7 (1: 0.3), the predominant polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the polyamine pattern showed the major compound putrescine. However, qualitative and quantitative differences in the major polyamine, polar lipid profile and fatty acid patterns distinguished strain Braz8T from species of the genus Thorsellia. Phylogenetic analysis based on 16S rRNA gene sequences, average nucleotide identity, DNA-DNA hybridization, multilocus sequence analysis as well as physiological and biochemical tests distinguished strain Braz8T both genotypically and phenotypically from the three Thorsellia species but also showed its placement in the family Thorselliaceae. Thus, strain Braz8T is considered to represent a novel species of a new genus most closely related to the genus Thorsellia, for which the name Coetzeea brasiliensis gen. nov., sp. nov. is proposed. The type strain of Coetzeea brasiliensis is Braz8T (=LMG 29552T=CIP 111088T). © 2016 IUMS. |
DOI: | 10.1099/ijsem.0.001497 |
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