Coetzeea brasiliensis gen. Nov., sp. nov. isolated from larvae of Anopheles darlingi

Resumo

A Gram-stain-negative, rod-shaped strain, Braz8T, isolated from larvae of Anopheles darlingi was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Braz8T was related most closely to species of the genus Thorsellia, with 95.6, 96.5 and 96.6% similarity to the type strains of Thorsellia anophelis, Thorsellia kandunguensis and Thorsellia kenyensis, respectively, and formed a separate branch in the phylogenetic tree next to the monophyletic cluster of the genus Thorsellia. Chemotaxonomic data supported the allocation of the strain to the family Thorselliaceae. The major fatty acids were C18:1ω7c, C16:0 and C14:0. The quinone system was composed of ubiquinones Q-8 and Q-7 (1: 0.3), the predominant polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the polyamine pattern showed the major compound putrescine. However, qualitative and quantitative differences in the major polyamine, polar lipid profile and fatty acid patterns distinguished strain Braz8T from species of the genus Thorsellia. Phylogenetic analysis based on 16S rRNA gene sequences, average nucleotide identity, DNA-DNA hybridization, multilocus sequence analysis as well as physiological and biochemical tests distinguished strain Braz8T both genotypically and phenotypically from the three Thorsellia species but also showed its placement in the family Thorselliaceae. Thus, strain Braz8T is considered to represent a novel species of a new genus most closely related to the genus Thorsellia, for which the name Coetzeea brasiliensis gen. nov., sp. nov. is proposed. The type strain of Coetzeea brasiliensis is Braz8T (=LMG 29552T=CIP 111088T). © 2016 IUMS.

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Dna, Bacterial, Cardiolipin, Fatty Acid, Nucleotide, Phosphatidylglycerol, Polyamine, Putrescine, Rna 16s, Ubiquinone, Dna, Bacterial, Fatty Acid, Phospholipid, Putrescine, Rna 16s, Ubiquinone, Ubiquinone 8, Anopheles Darlingi, Bacterium Isolation, Chemotaxonomy, Coetzeea Brasiliensis, Dna Hybridization, Fatty Acid Analysis, Gene Sequence, Gram Negative Bacterium, Larva, Multilocus Sequence Typing, Nonhuman, Phylogenetic Tree, Phylogeny, Priority Journal, Thorsellia Anophelis, Thorsellia Kandunguensis, Thorsellia Kenyensis, Type Strain, Animals, Anopheles, Bacterium Identification, Chemistry, Classification, Dna Base Composition, Dna Sequence, Enterobacteriaceae, Genetics, Isolation And Purification, Larva, Microbiology, Nucleic Acid Hybridization, Phylogeny, Animal, Anopheles, Bacterial Typing Techniques, Base Composition, Dna, Bacterial, Enterobacteriaceae, Fatty Acids, Larva, Nucleic Acid Hybridization, Phospholipids, Phylogeny, Putrescine, Rna, Ribosomal, 16s, Sequence Analysis, Dna, Ubiquinone

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