Please use this identifier to cite or link to this item: https://repositorio.inpa.gov.br/handle/1/15981
Title: In vitro and in vivo anti-malarial activity of limonoids isolated from the residual seed biomass from Carapa guianensis (andiroba) oil production
Authors: Pereira, Tiago B.
Silva, Luiz Francisco Rocha e
Amorim, Rodrigo C.N.
Melo, Márcia R.S.
Zacardi de Souza, Rita C.
Eberlin, M. N.
Lima, Emerson Silva
Vasconcellos, Marne Carvalho de
Pohlit, Adrian Martin
Keywords: 6alpha Acetoxyepoxyazadiradione
6alpha Acetoxygedunin
6alpha Hydroxydeacetylgedunin
7 Deacetoxy 7 Oxogedunin
Andirobin
Antimalarial Agent
Limonoid
Plant Extract
Unclassified Drug
Antimalarial Agent
Limonoid
Plant Extract
Animals Experiment
Animals Model
Antimalarial Activity
Carapa Guianensis
Controlled Study
Dose Response
Drug Cytotoxicity
Drug Efficacy
Drug Screening
Fibroblast
Human
Human Cell
Ic 50
In Vitro Study
In Vivo Study
Medicinal Plant
Meliaceae
Minimum Inhibitory Concentration
Mouse
Nonhuman
Seed Plant
Plasmodium Berghei Infection
Plasmodium Falciparum
Animals
Bagg Albino Mouse
Cell Line
Chemistry
Drug Effects
Female
Malaria
Meliaceae
Plasmodium Berghei
Animal
Antimalarials
Cell Line
Female
Humans
Inhibitory Concentration 50
Limonins
Malaria
Meliaceae
Mice
Mice, Inbred Balb C
Plant Extracts
Plasmodium Berghei
Plasmodium Falciparum
Seeds
Issue Date: 2014
metadata.dc.publisher.journal: Malaria Journal
metadata.dc.relation.ispartof: Volume 13, Número 1
Abstract: Background: Carapa guianensis is a cultivable tree used by traditional health practitioners in the Amazon region to treat several diseases and particularly symptoms related to malaria. Abundant residual pressed seed material (RPSM) results as a by-product of carapa or andiroba oil production. The objective of this study was to evaluate the in vitro and in vivo anti-malarial activity and cytotoxicity of limonoids isolated from C. guaianensis RPSM.Methods. 6-acetoxyepoxyazadiradione (1), andirobin (2), 6-acetoxygedunin (3) and 7-deacetoxy-7-oxogedunin (4) (all isolated from RPSM using extraction and chromatography techniques) and 6-hydroxy-deacetylgedunin (5) (prepared from 3) were evaluated using the micro test on the multi-drug-resistant Plasmodium falciparum K1 strain. The efficacy of limonoids 3 and 4 was then evaluated orally and subcutaneously in BALB/c mice infected with chloroquine-sensitive Plasmodium berghei NK65 strain in the 4-day suppressive test.Results: In vitro, limonoids 1-5 exhibited median inhibition concentrations (IC50) of 20.7-5.0 μM, respectively. In general, these limonoids were not toxic to normal cells (MRC-5 human fibroblasts). In vivo, 3 was more active than 4. At oral doses of 50 and 100 mg/kg/day, 3 suppressed parasitaemia versus untreated controls by 40 and 66%, respectively, evidencing a clear dose-response.Conclusion: 6-acetoxygedunin is an abundant natural product present in C. guianensis residual seed materials that exhibits significant in vivo anti-malarial properties. © 2014 Pereira et al.; licensee BioMed Central Ltd.x.
metadata.dc.identifier.doi: 10.1186/1475-2875-13-317
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