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Title: The JAK-STAT pathway controls Plasmodium vivax load in early stages of Anopheles aquasalis infection
Authors: Bahia, Ana Cristina
Kubota, Marina S.
Tempone, Antônio Jorge
Araújo, Helena R. C.
Guedes, Bruno A. M.
Orfanó, Alessandra Silva
Tadei, Wanderli Pedro
Ríos-Velásquez, Cláudia María
Han, Yeonsoo
Secundino, Nagilá Francinete Costa
Barillas-Mury, Carolina V.
Pimenta, Paulo Filemon Paolucci
Traub-Csekö, Yara Maria
Keywords: Janus Kinase
Nitric Oxide Synthase
Protein Inhibitor Of Activated Stat
Stat Protein
Nitric Oxide Synthase
Protein Inhibitor Of Activated Stat
Stat Protein
Anopheles Aquasalis
Controlled Study
Epithelium Cell
Gene Sequence
Gene Silencing
Nos Gene
Nucleotide Sequence
Organismal Interaction
Parasite Load
Pias Gene
Plasmodium Vivax
Protein Expression
Protozoal Genetics
Rna Interference
Stat Gene
Dna Sequence
Gene Expression Profiling
Isolation And Purification
Molecular Genetics
Gene Expression Profiling
Gene Knockdown Techniques
Molecular Sequence Data
Nitric Oxide Synthase
Plasmodium Vivax
Protein Inhibitors Of Activated Stat
Sequence Analysis, Dna
Stat Transcription Factors
Issue Date: 2011
metadata.dc.publisher.journal: PLoS Neglected Tropical Diseases
metadata.dc.relation.ispartof: Volume 5, Número 11
Abstract: Malaria affects 300 million people worldwide every year and 450,000 in Brazil. In coastal areas of Brazil, the main malaria vector is Anopheles aquasalis, and Plasmodium vivax is responsible for the majority of malaria cases in the Americas. Insects possess a powerful immune system to combat infections. Three pathways control the insect immune response: Toll, IMD, and JAK-STAT. Here we analyze the immune role of the A. aquasalis JAK-STAT pathway after P. vivax infection. Three genes, the transcription factor Signal Transducers and Activators of Transcription (STAT), the regulatory Protein Inhibitors of Activated STAT (PIAS) and the Nitric Oxide Synthase enzyme (NOS) were characterized. Expression of STAT and PIAS was higher in males than females and in eggs and first instar larvae when compared to larvae and pupae. RNA levels for STAT and PIAS increased 24 and 36 hours (h) after P. vivax challenge. NOS transcription increased 36 h post infection (hpi) while this protein was already detected in some midgut epithelial cells 24 hpi. Imunocytochemistry experiments using specific antibodies showed that in non-infected insects STAT and PIAS were found mostly in the fat body, while in infected mosquitoes the proteins were found in other body tissues. The knockdown of STAT by RNAi increased the number of oocysts in the midgut of A. aquasalis. This is the first clear evidence for the involvement of a specific immune pathway in the interaction of the Brazilian malaria vector A. aquasalis with P. vivax, delineating a potential target for the future development of disease controlling strategies. © 2011 Bahia et al.
metadata.dc.identifier.doi: 10.1371/journal.pntd.0001317
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