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Campo DC | Valor | Idioma |
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dc.contributor.author | Oliveira, Arlem Nascimento de | - |
dc.contributor.author | Oliveira, Luiz Antonio de | - |
dc.contributor.author | Andrade, Jerusa Souza | - |
dc.date.accessioned | 2020-06-02T20:08:34Z | - |
dc.date.available | 2020-06-02T20:08:34Z | - |
dc.date.issued | 2010 | - |
dc.identifier.uri | https://repositorio.inpa.gov.br/handle/1/16270 | - |
dc.description.abstract | Amylase production and partial characterization of crude enzyme preparations from two rhizobia strains (R-926 and R-991) were evaluated. For both the strains, maximal amylase activities were achieved during the early-to-midexponential growth phase; both were active over a pH range from 4.5 to 8.5 and temperature from 30 to 50 °C. None of the ions studied (K+, Na+, Ca2+, Hg2+, Mg2+, Mn2+, Cu2+ and Zn2+) was required for the catalytic activity of strain R-926; amylase activity of strain R-991 was stimulated in the presence of K+, Hg2+ and Zn2+. The surfactants SDS, Triton X-100 and Tween-80 did not have a pronounced inhibitory effect on enzyme activities; SDS and Tween-80 caused the highest stimulatory effects. Amylase activities from the rhizobia strains were reduced by up to 30% in the presence of EDTA; amylase activity of R-926 was also inhibited by HgCl2, suggesting that Ca2+and cysteine residues could be important for activity of this strain. | en |
dc.language.iso | en | pt_BR |
dc.relation.ispartof | Volume 53, Número 1, Pags. 35-45 | pt_BR |
dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Brazil | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/br/ | * |
dc.subject | Bradyrhizobium | en |
dc.subject | Rhizobiaceae | en |
dc.subject | Rhizobium | en |
dc.title | Partial characterization of amylases of two indigenous central amazonian rhizobia strains | en |
dc.type | Artigo | pt_BR |
dc.identifier.doi | 10.1590/S1516-89132010000100005 | - |
dc.publisher.journal | Brazilian Archives of Biology and Technology | pt_BR |
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