Use este identificador para citar ou linkar para este item:
https://repositorio.inpa.gov.br/handle/1/16305
Título: | Anti-Anopheles darlingi saliva antibodies as marker of Plasmodium vivax infection and clinical immunity in the Brazilian Amazon |
Autor: | Andrade, Bruno B. Rocha, Bruno Coelho Reis-Filho, Antonio Camargo, Luis Marcelo Aranha Tadei, Wanderli Pedro Moreira, Luciano Andrade Barral, Aldina Maria Prado Barral-Netto, M. |
Palavras-chave: | Darlingi Saliva Gland Sonicate Antibody Gamma Interferon Interleukin-10 Protozoon Antibody Unclassified Drug Antibody Biological Marker Gamma Interferon Insect Proteins Interleukin-10 Adult Antibody Blood Level Antibody Titer Blood Sampling Concentration (parameters) Control Group Controlled Study Cytokine Production Disease Severity Female Human Humoral Immunity Immunity Major Clinical Study Microscopy Parasitemia Plasmodium Vivax Plasmodium Vivax Malaria Polymerase Chain Reaction Screening Animals Anopheles Blood Immunology Middle Aged Plasmodium Vivax Malaria Prediction And Forecasting Saliva Adult Animal Anopheles Antibodies Biological Markers Female Humans Insect Proteins Interferon-gamma Interleukin-10 Malaria, Vivax Middle Aged Predictive Value Of Tests Saliva Young Adult |
Data do documento: | 2009 |
Revista: | Malaria Journal |
É parte de: | Volume 8, Número 1 |
Abstract: | Background. Despite governmental and private efforts on providing malaria control, this disease continues to be a major health threat. Thus, innovative strategies are needed to reduce disease burden. The malaria vectors, through the injection of saliva into the host skin, play important role on disease transmission and may influence malaria morbidity. This study describes the humoral immune response against Anopheles (An.) darlingi saliva in volunteers from the Brazilian Amazon and addresses the association between levels of specific antibodies and clinical presentation of Plasmodium (P.) vivax infection. Methods. Adult volunteers from communities in the Rondônia State, Brazil, were screened in order to assess the presence of P. vivax infection by light microscopy and nested PCR. Non-infected volunteers and individuals with symptomatic or symptomless infection were randomly selected and plasma collected. An. darlingi salivary gland sonicates (SGS) were prepared and used to measure anti-saliva antibody levels. Plasma interleukin (IL)-10 and interferon (IFN)- levels were also estimated and correlated to anti-SGS levels. Results. Individuals infected with P. vivax presented higher levels of anti-SGS than non-infected individuals and antibody levels could discriminate infection. Furthermore, anti-saliva antibody measurement was also useful to distinguish asymptomatic infection from non-infection, with a high likelihood ratio. Interestingly, individuals with asymptomatic parasitaemia presented higher titers of anti-SGS and lower IFN-/IL-10 ratio than symptomatic ones. In P. vivax-infected asymptomatic individuals, the IFN-/IL-10 ratio was inversely correlated to anti-SGS titers, although not for while in symptomatic volunteers. Conclusion. The estimation of anti-An. darlingi antibody levels can indicate the probable P. vivax infection status and also could serve as a marker of disease severity in this region of Brazilian Amazon. © 2009 Andrade et al; licensee BioMed Central Ltd. |
DOI: | 10.1186/1475-2875-8-121 |
Aparece nas coleções: | Artigos |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
---|---|---|---|---|
artigo-inpa.pdf | 256,06 kB | Adobe PDF | Visualizar/Abrir |
Este item está licenciada sob uma Licença Creative Commons