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Título: | Genomic Organization of Repetitive DNAs and Differentiation of an XX/XY Sex Chromosome System in the Amazonian Puffer Fish, Colomesus asellus (Tetraodontiformes) |
Autor: | Viana, Patrik Ferreira Ezaz, Tariq Tariq Marajó, Leandro Ferreira, Milena Zuanon, Jansen Cioffi, Marcelo de Bello Bertollo, Luiz Antônio Carlos Gross, Maria Claudia Feldberg, Eliana |
Palavras-chave: | Dna 18s Repetitive Dna Tropomyosin Cell Nucleus Antigen Nucleolar Organizer Region Associated Proteins Ribosome Dna Rna 18s Tropomyosin Acrocentric Chromosome C Banding Chromosome Nor Chromosome Number Cytogenetics Diploidy Female In Situ Hybridization, Fluorescence Heterochromatin Karyotype Male Nonhuman Priority Journal Puffer Fish Sex Chromosome Telomere X Chromosome Y Chromosome Animals Chromosome Banding Pattern Gene Amplification Genetics Nucleotide Repeat Sex Chromosome Sex Determination Processes Tetraodontiformes Ultrastructure Animal Antigens, Nuclear Chromosome Banding Ribosomal Dna Female Gene Amplification In Situ Hybridization, Fluorescence Male Repetitive Sequences, Nucleic Acid Rna, Ribosomal, 18s Sex Chromosomes Sex Determination Processes Telomere Tetraodontiformes Tropomyosin |
Data do documento: | 2018 |
Revista: | Cytogenetic and Genome Research |
É parte de: | Volume 153, Número 2, Pags. 96-104 |
Abstract: | The genus Colomesus is the sole representative of the family Tetraodontidae in the Amazon region. Here, Colomesus asellus was analyzed using conventional and molecular cytogenetic protocols. Its diploid chromosome number is 2n = 46 with 12 meta-, 10 submeta-, 16 subtelo-, and 8 acrocentric chromosomes and a fundamental number of FN = 84. An XX/XY sex chromosome system was identified. Mapping of 18S rDNA correlated with the nucleolus organizer regions (Ag-NORs) in the short arms of the 2 X chromosomes in females and in the Y chromosome in males. C-banding revealed heterochromatin in the centromeric regions of all chromosomes, except for pair 3. Prominent sex chromosome-specific heterochromatin amplification was observed, covering the short arms of the Y chromosome almost entirely. FISH with telomeric and tropomyosin (tpm1) sequences, respectively, revealed terminal signals in all chromosomes. The analysis of extended DNA fibers confirmed the colocalization and the interspersed pattern of the telomeric and tpm1 sequences. Thus, this study highlights the remarkable evolutionary dynamism presented by the Amazonian puffer fish regarding the differentiation of a heteromorphic XY sex chromosome system and a particular sex-specific amplification of rDNA sites. This is the first record of such an association in the Tetraodontidae family. © 2018 S. Karger AG, Basel. |
DOI: | 10.1159/000484423 |
Aparece nas coleções: | Artigos |
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