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Title: An RNA-based approach to sequence the mitogenome of Hypoptopoma incognitum (Siluriformes: Loricariidae)
Authors: Moreira, Daniel Andrade
Magalhães, Maithê Gaspar Pontes
Andrade, Paula C.C. de
Furtado, Carolina
Val, Adalberto Luis
Parente, Thiago E.M.
Keywords: Cytochrome C Oxidase
Cytochrome C Oxidase I
Cytochrome C Oxidase Ii
Phenylalanine Transfer Rna
Ribosome Rna
Rna 12s
Rna 16s
Unclassified Drug
Valine Transfer Rna
Fish Protein
Dna, Mitochondrial
Rna, Transfer
Gene Expression
Gene Sequence
Hypoptopoma Incognitum
Genome, Mitochondrial
Priority Journal
Start Codon
Stop Codon
Gene Order
Molecular Genetics
Rna Gene
Sequence Analysis
Whole Genome Sequencing
Codon, Terminator
Dna, Mitochondrial
Fish Proteins
Gene Order
Genes, Rrna
Genome, Mitochondrial
Molecular Sequence Annotation
Rna, Transfer
Sequence Analysis, Rna
Whole Genome Sequencing
Issue Date: 2016
metadata.dc.publisher.journal: Mitochondrial DNA
metadata.dc.relation.ispartof: Volume 27, Número 5, Pags. 3784-3786
Abstract: Hypoptopoma incognitum is a fish of the fifth most species-rich family of vertebrates and abundant in rivers from the Brazilian Amazon. Only two species of Loricariidae fish have their complete mitogenomes sequence deposited in the Genbank. An innovative RNA-based approach was used to assemble the complete mitogenome of H. incognitum with an average coverage depth of 5292×. The typical vertebrate mitochondrial features were found; 22 tRNA genes, two rRNA genes, 13 protein-coding genes, and a non-coding control region. Moreover, the use of this approach allowed the measurement of mtRNA expression levels, the punctuation pattern of editing, and the detection of heteroplasmies. © 2015 Taylor & Francis.
metadata.dc.identifier.doi: 10.3109/19401736.2015.1079903
Appears in Collections:Artigos

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