Use este identificador para citar ou linkar para este item: https://repositorio.inpa.gov.br/handle/1/17328
Título: Connarus favosus Planch.: An inhibitor of the hemorrhagic activity of Bothrops atrox venom and a potential antioxidant and antibacterial agent
Autor: Silva, Thaís Pereira da
Moura, Valéria Mourão de
Souza, Maria Carolina Scheffer de
Santos, Vanessa Neves Carvalho
Silva, Kiriaki Nurit M.Moreira da
Mendes, Mara Giselle Guimarães
Nunez, C. V.
Almeida, Patrícia Danielle Oliveira de
Lima, Emerson Silva
Mourão, Rosa Helena Veras
Santos, Maria Cristina dos
Palavras-chave: Antibiotic Agent
Antioxidant
Batroxobin
Connarus Favosus Extract
Phenanthroline
Phospholipase A2
Plant Extract
Tannin Derivative
Unclassified Drug
Antiinfective Agent
Antioxidant
Phospholipase A2
Plant Extract
Plant Medicinal Product
Snake Venom
Tannin Derivative
Venom Antiserum
Animals Experiment
Animals Model
Antibacterial Activity
Antioxidant Activity
Bleeding
Colorimetry
Controlled Study
Dpph Radical Scavenging Assay
Drug Cytotoxicity
Envenomation
Enzyme Activity
Female
Fibroblast
Hemolysis
Human
Human Cell
Ic 50
In Vitro Study
Male
Minimum Inhibitory Concentration
Mouse
Nonhuman
Phytochemistry
Electrophoresis, Polyacrylamide Gel
Protein Degradation
Quantitative Assay
Western Blotting
Zymography
Animals
Bothrops
Chemistry
Connaraceae
Hemorrhage
Metabolism
Procedures
Snake Bites
Medicine, Traditional
Animal
Anti-bacterial Agents
Antioxidants
Antivenins
Bothrops
Connaraceae
Crotalid Venoms
Female
Hemorrhage
Male
Medicine, Traditional
Mice
Phospholipases A2
Phytochemicals
Plant Extracts
Snake Bites
Tannins
Data do documento: 2016
Revista: Journal of Ethnopharmacology
É parte de: Volume 183, Pags. 166-175
Abstract: Ethnopharmacological relevance The plant species Connarus favosus is used in folk medicine in the west of Pará state, Brazil, to treat snakebites. Aim of the study To investigate the potential of the aqueous extract of Connarus favosus (AECf) to inhibit hemorrhagic and phospholipase A2 activities induced by Bothrops atrox venom (BaV) and to determine the antioxidant and antimicrobial potentials of the extract. Materials and methods AECf was analyzed phytochemically for phenolics (condensed tannins and hydrolyzable tannins) by colorimetry. Antioxidant activity was evaluated by quantitative assays using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Fe3+/phenanthroline. Antimicrobial activity was evaluated by the minimal inhibitory concentration test, and cytotoxicity was evaluated using human fibroblast cells (MRC-5). Inhibition of BaV-induced hemorrhagic activity was assessed after oral administration of the extract using pre-treatment, post-treatment and combined (BA plus AECf) treatment protocols. Inhibition of indirect hemolysis caused by phospholipase A2 (PLA2) was investigated in vitro. Interaction between AECf and BaV was investigated by SDS-PAGE electrophoresis, Western blot (Wb) and zymography. Results The phytochemical profile of AECf revealed ten secondary metabolite classes, and colorimetry showed high total phenolic and total (condensed and hydrolyzable) tannin content. AECf exhibited high antioxidant and antimicrobial potentials. The IC50 for the cytotoxic effect was 51.91 (46.86-57.50) μg/mL. Inhibition of BaV-induced hemorrhagic activity was significant in all the protocols, and inhibition of PLA2 activity was significant with the two highest concentrations. The BaV/AECf mixture produced the same bands as BaV by itself in SDS-PAGE and Wb although the bands were much fainter. Zymography confirmed the proteolytic activity of BaV, but when the venom was pre-incubated with AECf this activity was blocked. Conclusion AECf was effective in reducing BaV-induced hemorrhagic activity when administered by the same route as that used in folk medicine and exhibited antioxidant and antimicrobial potentials. © 2016 Elsevier Ireland Ltd. All rights reserved.
DOI: 10.1016/j.jep.2016.02.043
Aparece nas coleções:Artigos

Arquivos associados a este item:
Não existem arquivos associados a este item.


Os itens no repositório estão protegidos por copyright, com todos os direitos reservados, salvo quando é indicado o contrário.