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Title: Efficient and safe gene transfection in fish spermatogonial stem cells using nanomaterials
Authors: Tonelli, Fernanda MP
Lacerda, Samyra Maria Santos N.Nassif
Paiva, N. C O
Lemos, Moline Severino
Jesus, A. C. de
Pacheco, Flávia G.
Corrêa-Junior, José D.
Ladeira, Luiz Orlando
Furtado, Clascídia Aparecida
França, Luiz Renato de
Resende, Rodrigo Ribeiro
Keywords: Carbon
Cell Death
Gene Transfer
Multiwalled Carbon Nanotubes (mwcn)
Nanostructured Materials
Stem Cells
Commercial Reagents
Gene Delivery
Gene Transfection
Gold Nanorod
Multiwalled Carbon Nanotube (mwcnts)
Nile Tilapia
Molecular Biology
Issue Date: 2016
metadata.dc.publisher.journal: RSC Advances
metadata.dc.relation.ispartof: Volume 6, Número 58, Pags. 52636-52641
Abstract: Multiwalled carbon nanotubes (MWCNTs), nanographene oxide (NGO), and gold nanorods (NRs) can be functionalized and complexed to DNA to promote efficient gene delivery to Nile tilapia spermatogonial stem cells inducing less cell death than electroporation and the commercial reagents tested. Therefore, nanomaterials can contribute to achieve fish transgenesis. © The Royal Society of Chemistry.
metadata.dc.identifier.doi: 10.1039/c6ra07010h
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