Use este identificador para citar ou linkar para este item: https://repositorio.inpa.gov.br/handle/1/17680
Título: Gastro-protective effects of isobrucein B, a quassinoid isolated from Picrolemma sprucei
Autor: Manfredo Vieira, Silvio
Silva, Rangel Leal
Lemos, Henrique Paula
Amorim, Rodrigo C.N.
Silva, Ellen Cristina Costa
Reinach, Peter Sol
Cunha, Fernando Queiroz
Pohlit, Adrian Martin
Cunha, Thiago Mattar
Palavras-chave: Cxcl1 Chemokine
Interleukin-1beta
Isobrucein B
Nonsteroid Antiinflammatory Agent
Prostaglandin E2
Prostaglandin Synthase
Protective Agent
Quassinoid Derivative
Tumor Necrosis Factor-alpha
Unclassified Drug
Cytokine
Indometacin
Isobrucein B
Nonsteroid Antiinflammatory Agent
Plant Extract
Prostaglandin E2
Quassinoid Derivative
Adult
Animals Experiment
Animals Model
Animals Tissue
Capillary Wall
Carbon Nuclear Magnetic Resonance
Controlled Study
Drug Mechanism
Enzyme-linked Immunosorbent Assay
Gastritis
Leukocyte Adherence
Leukocyte Migration
Leukocyte Rolling
Male
Microcirculation
Mouse
Neutrophil Chemotaxis
Nonhuman
Pathophysiology
Picrolemma sprucei
Plant Root
Plant Stem
Priority Journal
Prostaglandin Synthesis
Protection
Proton Nuclear Magnetic Resonance
Simaroubaceae
Stomach Injury
Stomach Lesion
Animals
C57bl Mouse
Cell Adhesion
Chemical Structure
Chemically Induced
Chemistry
Disease Model
Dose Response
Drug Effects
Gastritis
Isolation And Purification
Leukocyte
Medicinal Plant
Metabolism
Neutrophil
Phytotherapy
Simaroubaceae
Stomach Mucosa
Animal
Anti-inflammatory Agents, Non-steroidal
Cell Adhesion
Cytokines
Dinoprostone
Disease Models, Animals
Dose-response Relationship, Drug
Gastric Mucosa
Gastritis
Indomethacin
Leukocytes
Male
Mice
Mice, Inbred C57bl
Molecular Structure
Neutrophils
Phytotherapy
Plant Extracts
Plant Roots
Plant Stems
Plants, Medicinal
Quassins
Simaroubaceae
Data do documento: 2014
Revista: Fitoterapia
É parte de: Volume 95, Pags. 8-15
Abstract: Infusions of Picrolemma sprucei roots, stems and leaves are used in traditional medicine throughout the Amazon region from the Guianas to Brazil and Peru in the treatment of gastritis, intestinal helminths and malaria. As there are no studies describing its mode of action in providing a gastroprotective effect, we determined herein that one of the main constituents found in P. sprucei infusions, the quassinoid isobrucein B (IsoB), reduces some of the pathophysiological effects in a mouse model of non-steroidal anti-inflammatory drug (NSAID)-induced gastritis and provides mechanisms of action. Then, IsoB (1.17 g) was isolated from the roots and stems (6.5 kg) of P. sprucei. Its structure was confirmed by 1D and 2D 1H and 13C NMR, ESI-tof-MS, IR and UV. C57BL/6 strain mice were subcutaneously injected with IsoB (0.5-5 mg kg- 1) or vehicle before oral administration of indomethacin and sacrificed later at different time points. Gastric damage was assessed by measuring lesion length. Leukocyte migration was evaluated based on leukocyte rolling and adhesion using intravital microscopy in the mesenteric microcirculation and tissue MPO activity. Stomach extract cytokine (TNFα, IL-1β and KC/CXCL1) and prostaglandin E2 (PGE2) levels were measured by ELISA and RIA, respectively. IsoB pre-treatment (0.5-5.0 mg kg- 1) significantly reduced the formation of indomethacin-induced stomach lesions in a dose-dependent manner. The decrease in stomach lesions was associated with less observed leukocyte rolling, decreased leukocyte adhesion and less neutrophil infiltration (MPO activity). IsoB (1 mg kg- 1) pre-treatment did not prevent indomethacin-induced decreases in stomach PGE2 levels. However, IL-1β and KC/CXCL1 levels were inhibited by this same IsoB dosage, whereas TNF-α was unchanged. IsoB may be a prototypic compound to provide protective effects against NSAID-induced gastritis and possibly other gastropathies. Moreover, IsoB gastroprotective action may be due to a reduction in IL-1β and KC/CXCL1 production/release and leukocyte rolling, adhesion and migration. © 2014 Elsevier B.V.
DOI: 10.1016/j.fitote.2014.02.008
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