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https://repositorio.inpa.gov.br/handle/1/17981| Title: | Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building |
| Authors: | David, Susana Correia de Matos Sutre, Ana Filipa Sanca, A. Mané, A. Henriques, V. Portugal, Clara Sancho, Luísa Cardoso, Ângela Paixão, Eleonora Duarte, Elsa Leclerc Leite, Clarice Queico Fujimura Salem, Júlia Ignez Antunes, Abílio |
| Keywords: | Double Stranded Dna Sodium Hypochlorite Rifampicin Antibiotic Resistance Bacterial Gene Controlled Study Denaturing High Performance Liquid Chromatography Dna Extraction Gene Frequency Gene Identification Gene Locus Gene Mutation Guinea-bissau Human Lung Tuberculosis Major Clinical Study Molecular Diagnosis Mutational Analysis Polymerase Chain Reaction Priority Journal Rpob Gene Sequence Analysis Polymorphism, Single Nucleotide Sputum Smear Antitubercular Agents Bacterial Proteins Base Sequence Capacity Building Clinical Laboratory Techniques Guinea-bissau Humans Laboratories Microbial Sensitivity Tests Mycobacterium Tuberculosis Portugal Rifampin Sequence Analysis Sodium Hypochlorite Specimen Handling Sputum Tuberculosis |
| Issue Date: | 2012 |
| metadata.dc.publisher.journal: | International Journal of Tuberculosis and Lung Disease |
| metadata.dc.relation.ispartof: | Volume 16, Número 11, Pags. 1535-1537 |
| Abstract: | The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings. © 2012 The Union. |
| metadata.dc.identifier.doi: | 10.5588/ijtld.11.0658 |
| Appears in Collections: | Artigos |
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