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Title: Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil
Authors: Freire, Ana Karla Lima L.
Bentes, Amaury dos Santos
Lima Sampaio, Ivanete de
Matsuura, Ani Beatriz Jackisch
Ogusku, Maurício Morishi
Salem, Júlia Ignez
Wanke, Bodo
Souza, João Vicente Braga de
Keywords: Fungal Protein
Unclassified Drug
Ura5 Protein
Cerebrospinal Fluid
Controlled Study
Cryptococcus Gattii
Cryptococcus Neoformans
Dna Fingerprinting
Fungal Gene
Fungus Isolation
Gene Expression
Gene Locus
Healthcare Facility
Human Immunodeficiency Virus
Human Immunodeficiency Virus Infection
Major Clinical Study
Molecular Genetics
Polymerase Chain Reaction
Priority Journal
Polymorphism, Restriction Fragment Length
Risk Factor
Species Diversity
Tertiary Health Care
Variable Number Of Tandem Repeat
Virus Isolation
Ambulatory Care Facilities
Child, Preschool
Middle Aged
Molecular Sequence Data
Mycological Typing Techniques
Polymorphism, Restriction Fragment Length
Young Adult
Cryptococcus Gattii
Filobasidiella Neoformans
Issue Date: 2012
metadata.dc.publisher.journal: Mycoses
metadata.dc.relation.ispartof: Volume 55, Número 3, Pags. e145-e150
Abstract: As there are four major molecular types of Cryptococcus neoformans (VNI, VNII, VNIII and VNIV) and four molecular types of Cryptococcus gattii (VGI, VGII, VGIII and VGIV), it is important to identify the specific groups causing cryptococcosis in different geographical regions. Here, we investigated the molecular types of 57 cryptococcal isolates from patients in a tertiary care hospital in the state of Amazonas, Brazil, between 2006 and 2010. The isolates were characterised by PCR fingerprinting using the M13 minisatellite and confirmed by URA5-RFLP analysis, and the presence of specific genes from the mating type locus (MATα and MATa) of these species was analysed by PCR. Most of the patients were male (66.7%), between 16 and 30years of age (51.7%), and HIV-positive (75.0%). Most isolates were collected from cerebrospinal fluid samples (71.7%). Most of the C. neoformans isolates (n=40) were characterised as members of the VNI molecular group (n=39), a unique isolate was characterised as VNII whereas all isolates of C. gattii (n=17) were members of the VGII molecular group. With regard to mating types, 55 isolates were type 'α', and only two were type 'a'. This study revealed the prevalence of the VNI molecular group and provides the first reported observation of the VNII molecular group in the northern region of Brazil. © 2012 Blackwell Verlag GmbH.
metadata.dc.identifier.doi: 10.1111/j.1439-0507.2012.02173.x
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