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https://repositorio.inpa.gov.br/handle/1/18167| Title: | Microsatellites for mahoganies: Twelve new loci for Swietenia macrophylla and its high transferability to Khaya senegalensis |
| Authors: | Lemes, Maristerra R. Esashika, Thana Gaoué, Orou Gandé |
| Keywords: | Microsatellite Dna Dna, Plant Allele Conservation Genetics Endangered Species Evergreen Tree Gene Flow Gene Transfer Genetic Marker Heterozygosity Reproductive Strategy Timber Tropical Environment Allele Endangered Species Gene Frequency Gene Locus Polymorphism, Genetic Genetics Heterozygote Heterozygote Detection Inheritance Meliaceae Methodology Plant Leaf Electrophoresis, Polyacrylamide Gel Polymerase Chain Reaction Species Difference Alleles Dna, Plant Electrophoresis, Polyacrylamide Gel Endangered Species Gene Frequency Genetic Loci Heterozygote Heterozygote Detection Inheritance Patterns Meliaceae Microsatellite Repeats Plant Leaves Polymerase Chain Reaction Polymorphism, Genetic Species Specificity Khaya Senegalensis Meliaceae Swietenia Swietenia Macrophylla |
| Issue Date: | 2011 |
| metadata.dc.publisher.journal: | American Journal of Botany |
| metadata.dc.relation.ispartof: | Volume 98, Número 8, Pags. e207-e209 |
| Abstract: | Premise of the study: A new set of 12 microsatellite markers was developed and characterized for big-leaf mahogany (Swietenia macrophylla King, Meliaceae) and its transferability assayed in the African mahogany, Khaya senegalensis (Desr.) A. Juss. (Meliaceae), to study population and conservation genetics of these threatened tropical timber species. Methods and Results: Using an enriched library approach twelve novel microsatellite loci were identified for S. macrophylla. The number of alleles per locus ranged from 5 to 14 and mean observed and expected heterozygosities were 0.819 and 0.822, respectively. Twenty microsatellite loci developed for S. macrophylla (12 from this study and eight previously published) were tested for K. senegalensis and 10 polymorphic were characterized. Conclusions: The results show the highly informative content of the new SSR loci for Swietenia macrophylla and the high effectiveness of these microsatellites for population genetics, gene flow, and mating system studies in Khaya senegalensis. © 2011 Botanical Society of America. |
| metadata.dc.identifier.doi: | 10.3732/ajb.1100074 |
| Appears in Collections: | Artigos |
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