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Title: Formulation of a liquid medium with wheat bran for the production of laccase by Trametes versicolor in an air-lift bioreactor
Authors: Souza, João Vicente Braga de
Silva, Érica M.
Cavallazzi, José Renato Pereira
Sobrinho, Agostinho da S.
Keywords: Copper Sulfate
Aeration And Oxygenation
Concentration (parameters)
Controlled Study
Drug Formulation
Enzyme Activity
Enzyme Synthesis
Liquid Culture
Ph Measurement
Trametes Versicolor
Wheat Bran
Trametes Versicolor
Triticum Aestivum
Issue Date: 2010
metadata.dc.publisher.journal: Journal of Food, Agriculture and Environment
metadata.dc.relation.ispartof: Volume 8, Número 1, Pags. 394-396
Abstract: The aim of this work was to formulate a liquid medium with wheat bran for the production of Trametes versicolor laccase in an air-lift bioreactor. Univariated experiments were carried out in order to optimize the contents of CuSO4 (0-2 mM), glucose (0-50 g/L), peptone (0-25 g/L) and fermentation parameters as pH (3-7), inoculum (40-360 mycelium plugs/L) and aeration (0-1 v/v/m). After the optimization an optimized experiment was accomplished (pH 3, inoculum of 40 mycelium plugs/L, CuSO4 concentration of 1.2 mM, 10 g/L of peptone and the aeration of 0.5 v/v/m) in an airlift reactor. The highest activity (32,000 UI/L) was reached in the 10th experimental day.
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