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dc.contributor.authorHonda, Rubens Tomio-
dc.contributor.authorAraújo, Roziete Mendes-
dc.contributor.authorHorta, Bruno Brasil-
dc.contributor.authorVal, Adalberto Luis-
dc.contributor.authorDemasi, Marilene-
dc.date.accessioned2020-06-15T22:03:21Z-
dc.date.available2020-06-15T22:03:21Z-
dc.date.issued2005-
dc.identifier.urihttps://repositorio.inpa.gov.br/handle/1/18836-
dc.description.abstractWe describe a one-step purification of hepatic metallothionein from the Amazon fish Colossoma macropomum injected with cadmium and from the copper-loaded metallothionein from the yeast Saccharomyces cerevisiae, performed by affinity chromatography through metal-chelating columns. Yeast metallothionein was purified from Cu2+-loaded resin and eluted by a continuous EDTA gradient whereas hepatic metallothionein extracted from fishes was purified by Ni2+-loaded resin and eluted by a continuous imidazol gradient. Purified metallothioneins were evaluated by SDS-PAGE and characterized by UV spectra of the apo- and Cd2+-loaded protein. This method allowed high purity and yield as well as rapid one-step extraction of both metal-loaded and apoprotein. © 2005 Elsevier B.V. All rights reserved.en
dc.language.isoenpt_BR
dc.relation.ispartofVolume 820, Número 2, Pags. 205-210pt_BR
dc.rightsRestrito*
dc.subjectChelationen
dc.subjectChromatographic Analysisen
dc.subjectExtractionen
dc.subjectProteinsen
dc.subjectPurificationen
dc.subjectUltraviolet Spectroscopyen
dc.subjectYeasten
dc.subjectApoproteinsen
dc.subjectMetal-chelating Columnsen
dc.subjectMetallothioneinen
dc.subjectSaccharomyces Cerevisiaeen
dc.subjectOrganometallicsen
dc.subjectApoproteinen
dc.subjectCadmiumen
dc.subjectCopperen
dc.subjectEdetic Aciden
dc.subjectImidazoleen
dc.subjectMetallothioneinen
dc.subjectResinen
dc.subjectChromatography, Affinityen
dc.subjectAnimals Experimenten
dc.subjectAnimals Tissueen
dc.subjectChemical Proceduresen
dc.subjectColossoma Macroponumen
dc.subjectControlled Studyen
dc.subjectElutionen
dc.subjectExtractionen
dc.subjectFishen
dc.subjectNonhumanen
dc.subjectElectrophoresis, Polyacrylamide Gelen
dc.subjectPriority Journalen
dc.subjectProtein Purificationen
dc.subjectSaccharomyces Cerevisiaeen
dc.subjectUltraviolet Spectroscopyen
dc.subjectYeasten
dc.subjectAnimalen
dc.subjectElectrophoresis, Polyacrylamide Gelen
dc.subjectFishesen
dc.subjectMetallothioneinen
dc.subjectSpectrophotometry, Ultravioleten
dc.subjectColossoma Macropomumen
dc.subjectPiscesen
dc.subjectSaccharomyces Cerevisiaeen
dc.titleOne-step purification of metallothionein extracted from two different sourcesen
dc.typeArtigopt_BR
dc.identifier.doi10.1016/j.jchromb.2005.03.017-
dc.publisher.journalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciencespt_BR
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