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Title: Two main clusters within Trypanosoma cruzi zymodeme 3 are defined by distinct regions of the ribosomal RNA cistron
Authors: Mendonça, M. B A
Nehme, Nédia Saad
Santos, Silvana Eloi Silva
Cupolillo, Elisa
Vargas, Nancy S.
Junqueira, Ângela Cristina Veríssimo
Naiff, Roberto Daibes
Barrett, Toby Vincent
Coura, José Rodrigues
Zingales, Bianca S.
Fernandes, Octávio
Keywords: Endonuclease
Ribosome Dna
Ribosome Rna
Unclassified Drug
Zymodeme 3
Controlled Study
Gene Cluster
Genetic Conservation
Genetic Organization
Genetic Variability
Nucleotide Sequence
Parasite Isolation
Polymerase Chain Reaction
Priority Journal
Protein Domain
Polymorphism, Restriction Fragment Length
Southern Blotting
Trypanosoma Cruzi
Southern Blotting
Chagas Disease
Dna, Protozoan
Dna, Ribosomal Spacer
Electrophoresis, Agar Gel
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Rna, Ribosomal
Trypanosoma Cruzi
Variation (genetics)
Panstrongylus Geniculatus
Rhodnius Brethesi
Trypanosoma Cruzi
Issue Date: 2002
metadata.dc.publisher.journal: Parasitology
metadata.dc.relation.ispartof: Volume 124, Número 2, Pags. 177-184
Abstract: Trypanosoma cruzi is currently classified into 2 major phylogenetic lineages, T. cruzi I and II, that correlate with the formerly described zymodeme 1 and 2, respectively. Another isoenzymic group (zymodeme 3-Z3) was also described. In this study, we analysed the genetic diversity among Z3 isolates of the Brazilian Amazon by restriction fragment length polymorphism of the intergenic transcribed spacers (ITSs) of the ribosomal RNA cistron and the size of the divergent domain D7 of the 24Sα rRNA gene. DNAs from 12 T. cruzi Z3 isolates obtained from humans (2), Panstrongylus geniculatus (1), and Rhodnius brethesi (9) were submitted to PCR amplification of the ITSs plus the 5.8S rDNA. The PCR products were digested with 4 distinct endonucleases and the profiles analysed by a numerical methodology. The phenetic dendrogram revealed a clear dichotomy in the Z3 group, defining 2 groups that were named Z3-A and Z3-B. Dimorphism was also found in the band sizes of the amplified D7 divergent domain of the 24Sα rDNA, which showed a perfect correlation with the ITSs clustering. The organization of the ribosomal cistron was investigated by Southern blotting and shown to be conserved in the genome of the 2 Z3 groups. This study shows that the rDNA cistron allows the definition of 2 distinct subclusters in Z3 isolates.
metadata.dc.identifier.doi: 10.1017/S0031182001001172
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