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Title: Physical mapping of the anopheles (Nyssorhynchus) darlingi genomic scaffolds
Authors: Rafael, Míriam Silva
Bridi, Letícia Cegatti
Sharakhov, Igor V.
Marinotti, Osvaldo
Sharakhova, Maria V.
Timoshevskiy, Vladimir A.
Guimarães-Marques, Giselle Moura
Santos, Valéria Silva
da Silva, Carlos Gustavo Nunes
Astolfi-Filho, Spártaco A.T.
Tadei, Wanderli Pedro
Keywords: in situ hybridization
genomics and cytogenetics
polytene chromosome
Issue Date: 2021
metadata.dc.publisher.journal: Insects
metadata.dc.relation.ispartof: Volume 12, Número 2, págs. 1-12
Abstract: The genome assembly of Anopheles darlingi consists of 2221 scaffolds (N50 = 115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of the smallest genomes among Anopheles species. Anopheles darlingi genomic DNA fragments of ~37 Kb were cloned, end-sequenced, and used as probes for fluorescence in situ hybridization (FISH) with salivary gland polytene chromosomes. In total, we mapped nine DNA probes to scaffolds and autosomal arms. Comparative analysis of the An. darlingi scaffolds with homologous sequences of the Anopheles albimanus and Anopheles gambiae genomes identified chromosomal rearrangements among these species. Our results confirmed that physical mapping is a useful tool for anchoring genome assemblies to mosquito chromosomes.
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