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Title: Variabilidade genética espacial e temporal de Aedes (Stegomyia) aegypti (Linnaeus, 1762) em Manaus-AM, com o emprego de marcadores microssatélites
Authors: Mendonça, Bárbara Alessandra Alves
metadata.dc.contributor.advisor: Scarpassa, Vera Margarete
Keywords: Aedes aegypti
Estrutura microgeográfica
Genética populacional
Issue Date: 9-Aug-2010
Publisher: Instituto Nacional de Pesquisa da Amazônia - INPA
metadata.dc.publisher.program: Genética, Conservação e Biologia Evolutiva - GCBEv
metadata.dc.description.resumo: Aedes aegypti is the vector of dengue. Most important arbovirus in the news, reaching the human populations in tropical and subtropical areas of the globe, coupled with high rates of morbidity and mortality. To date, no vaccine is available and no effective treatment to patients who get one of their serotypes (DENV-1, DENV-2, DENV-3 or DENV-4). Thus, the most effective prophylactic tool for reducing the transmission rates is the use of integrated control of its vector. Thus, we performed a genetic study time, to better understand their population dynamics, which is related to environmental factors. Thus, populations of this mosquito were analyzed from four locations in Manaus-AM (Jorge Teixeira and Coroado - East Zone, Cachoerinha, Centro and Praça 14 de Janeiro - South and Cidade Nova - North Zone), collected during the rainy and dry seasons. At first, 165 individuals were obtained in the second, 156 individuals. After processing the specimens (establishment in insectary, identification and freezing in a freezer at -80°C), were performed extractions and amplifications of their DNA, using dinucleotide microsatellite loci. His alleles were visualized on polyacrylamide gel 6%, stained with silver nitrate and analyzed data on specific statistical programs. In both seasons, the heterozigosidase expected (HE) average was higher in eastern zone and ranged from 0.555 to 0.613 in Coroado Crown (rainy season) and from 0.530 to 0.594 in Jorge Teixeira (dry season). The FST values and hierarchical analysis (AMOVA) showed low genetic structure among samples of the rainy season. However, there was low and significant structure among some samples of the dry season. These results were supported by low values of effective population size (Ne) in the dry season, following the model of linkage disequilibrium. The Bayesian analysis of the program Structure showed the existence of a reverse split in the four locations and at both stations (K = 1). The Mantel test showed no isolation by distance between locations in both seasons. The presence of a significant structure in the dry season may have been due to the reduction of breeding sites available for oviposition, with subsequent reduction in population densities of Ae. aegypti and therefore the effective population size, with loss of genetic variability and genetic structure formation more evident during the dry season.
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