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Title: Citogenética comparativa de seis espécies de anuros do gênero Leptodactylus (Leptodactylidae) coletadas no estado do Amazonas, Brasil
Authors: Coelho, Ana Carolina
metadata.dc.contributor.advisor: Gross, Maria Claudia Menin, Marcelo
Keywords: Anuros
Issue Date: 27-Mar-2013
Publisher: Instituto Nacional de Pesquisa da Amazônia - INPA
metadata.dc.publisher.program: Genética, Conservação e Biologia Evolutiva - GCBEv
metadata.dc.description.resumo: The genus Leptodactylus has 75 species, most of them are from Neotropical, and with a modal diploid number of 22 chromosomes. However, cytogenetics analyses are incipient among Leptodactylus species from Central Amazon and studies about the karyotype pattern of the five intrageneric groups are poorly known (L. fuscus, L. marmoratus, L. melanonotus, L. ocellatus, L. pentadactylus). Classical and molecular cytogenetic analyses of mitotic chromosomes were conducted on five species of Leptodactylus from Central Amazon (L. andreae, L. macrosternum, L. pentadactylus, L. petersii and L. riveroi), and meiotic analyses for species of L. hylaedactylus and L. petersii. The species L. andreae and L. hylaedactylus showed 2n=26 chromosomes and other species which were analyzes showed 2n=22 chromosomes. The nucleolus organizer region (NOR) was evident in the pair 8 of L. andreae, L. macrosternum, L. pentadactylus and L. riveroi, while L. petersii was evident in the pairs 6 and 10. These results of NOR were confirmed by 18S rDNA probe, except for the pair 10 of L. petersii. The C-banding pattern was evident in the centromeric region of the five species, which were studied mitotically, and species-specific variations were observed in this present study. Regarding meiotic analyzes, L. hylaedactylus showed 13 bivalents of metaphasis I while L. petersii showed 11 bivalents in the same phase. No intersticial telomeric site was evident, however doesn’t mean that no rearrangements occurred during karioevolution of this genus, since variations in karyotype formula were observed both intraspecific and interespecific. Respect to standard karyotypics of intrageneric groups, diploid number as equal as 22 chromosomes were observed in L. ocellatus species as well as into the groups of L. fuscus and L. pentadactylus. In the group L. melanonotus, three diploid numbers were presented: 20, 22 and 24, and the most variation of diploid number were observed in the L. marmoratus group. These diploid numbers combined with data from constitutive heterochromatin standard distribution and organized nucleolus region, which are available for several species of genus Leptodactylus, inficate that L. ocellatus group would be ancient and L. marmoratus would be the newest, corroborating the proposal based in reproductive traits.
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