Mapeamento cromossômico comparativo de Saguinus bicolor e Saguinus midas utilizando sequências repetitivas de DNA
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Serfaty, Dayane Martins Barbosa
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Schneider, Carlos Henrique
Coorientador(a)
Gordo, Marcelo
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Instituto Nacional de Pesquisa da Amazônia - INPA
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Saguinus is the largest and most complex genus of the subfamily Callitrichinae,
with 23 species. They are distributed from Southern of Central America to
northern of South America. Saguinus bicolor have very limited geographic
distribuition, affected by demographic expansion of the city Manaus. In contrast,
Saguinus midas have largest geographic distribuition among the Saguinus.
They share the same characteristics general and overlap the north of Manaus.
Cytogenetics studies with Saguinus described a karyotypic macrostructure
conserved, with 2n=46 and patterns of similar bands. However, mapping
studies of repetitive sequence are incipient. Repetitive sequence in tandem:
telomere and rDNA; and repetitive sequence dispersed include the
transposable elements were searched in the work. Analysis were made on S.
midas and two populations of S. bicolor. The classical cytogenetics confirmed
macrostructure of 2n=46, but differed in morphology of chromosomes, classified
into: 8 metacentrics; 10 submetacentrics; 10 subtelocentrics and 6 acrocentrics.
The patterns bands were similar, but showed variations among individuals of
the same species. The G-bands patterns suggest the fourth pair as cytogenetic
markers that show differences among two species and identify natural hybrids
in contact zone. The NOR’s were detected in pairs 17 and 18, agreeing with the
localization of sequences of rDNA 18S in region pericentromeric of long arms of
chromosomes 17, 18 and 19, located in heterochomatic region. LINE–1 was
found in regions: euchromatics – having an impact on the organization and
function of genome, and; heterochromatics - particularly in centromeric
heterochromatin. Accumulation in sex chromosomes are associated with
inactivation of one chromosome X in females to promote the gene silencing and
ensure gene dosage of sex pair when compared with male. It is possible to
observe his presence in regions of negatives G-bands (light bands) implying
that deposition in genome of S. bicolor and S. midas is recent in evolutionary
time. Differences of sinalization of LINE-1 among populations of S. bicolor were
detected, possibly due to isolation the two populations.
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