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dc.contributor.authorOliveira, C. V.S. de-
dc.contributor.authorMatos, Kedma Silva-
dc.contributor.authorAlbuquerque, Dulcinéia Martins C. de-
dc.contributor.authorHanada, Rogério Eiji-
dc.contributor.authorSilva, Gilvan Ferreira da-
dc.description.abstractChili pepper (Capsicum chinense) is a great economic important culture on the State of Amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. It is one of the hosts of Colletotrichum genus in the North region of Brazil. The aim of the study was to differentiate and to identify isolates of Colletotrichum collected from C. chinense in Amazon. Molecular characterization, using RFLP-PCR, ERIC-PCR and ISSR, was carried out initially for screening of morphologically similar isolates. Furthermore, phylogenetic analyses were performed using combined regions: Actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for the three isolates, INPA 2066, INPA 2286 and INPA 1858, plus superoxide dismutase (SOD2) for INPA 2066. We showed that the molecular markers were able to distinguish the isolates of Colletotrichum studied and these results were confirmed with the phylogenetic analyses, three different occurrences of Colletotrichum species (C. siamense, C. scovillei and C. brevisporum) causing anthracnose in C. chinense in the State of Amazonas. This study represents the first report of the species C. siamense and C. scovillei in this host in Brazil. © 2017 The Authors.en
dc.relation.ispartofVolume 16, Número 2pt_BR
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Brazil*
dc.subjectGlyceraldehyde 3 Phosphate Dehydrogenaseen
dc.subjectMolecular Markeren
dc.subjectSuperoxide Dismutaseen
dc.subjectCapsicum Chinenseen
dc.subjectColletotrichum Brevisporumen
dc.subjectColletotrichum Scovilleien
dc.subjectColletotrichum Siamenseen
dc.subjectControlled Studyen
dc.subjectCrop Productionen
dc.subjectFungus Identificationen
dc.subjectFungus Isolationen
dc.subjectInter Simple Sequence Repeaten
dc.subjectNucleotide Sequenceen
dc.subjectPolymerase Chain Reactionen
dc.subjectPolymorphism, Restriction Fragment Lengthen
dc.subjectUnindexed Sequenceen
dc.titleIdentification of Colletotrichum isolates from Capsicum chinense in Amazonen
dc.publisher.journalGenetics and Molecular Researchpt_BR
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