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Title: Identification of Colletotrichum isolates from Capsicum chinense in Amazon
Authors: Oliveira, C. V.S. de
Matos, Kedma Silva
Albuquerque, Dulcinéia Martins C. de
Hanada, Rogério Eiji
Silva, Gilvan Ferreira da
Keywords: Actin
Glyceraldehyde 3 Phosphate Dehydrogenase
Molecular Marker
Superoxide Dismutase
Capsicum Chinense
Colletotrichum Brevisporum
Colletotrichum Scovillei
Colletotrichum Siamense
Controlled Study
Crop Production
Fungus Identification
Fungus Isolation
Inter Simple Sequence Repeat
Nucleotide Sequence
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Unindexed Sequence
Issue Date: 2017
metadata.dc.publisher.journal: Genetics and Molecular Research
metadata.dc.relation.ispartof: Volume 16, Número 2
Abstract: Chili pepper (Capsicum chinense) is a great economic important culture on the State of Amazonas, and it represents, approximately, a production of 1.9 thousand tons per year. It is one of the hosts of Colletotrichum genus in the North region of Brazil. The aim of the study was to differentiate and to identify isolates of Colletotrichum collected from C. chinense in Amazon. Molecular characterization, using RFLP-PCR, ERIC-PCR and ISSR, was carried out initially for screening of morphologically similar isolates. Furthermore, phylogenetic analyses were performed using combined regions: Actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for the three isolates, INPA 2066, INPA 2286 and INPA 1858, plus superoxide dismutase (SOD2) for INPA 2066. We showed that the molecular markers were able to distinguish the isolates of Colletotrichum studied and these results were confirmed with the phylogenetic analyses, three different occurrences of Colletotrichum species (C. siamense, C. scovillei and C. brevisporum) causing anthracnose in C. chinense in the State of Amazonas. This study represents the first report of the species C. siamense and C. scovillei in this host in Brazil. © 2017 The Authors.
metadata.dc.identifier.doi: 10.4238/gmr16029601
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